Sinoaortic denervation alters the molecular and endocrine responses to salt loading.
Sinoaortic denervation alters the molecular and endocrine responses to salt loading.
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主动脉去神经改变了分子和内分泌对盐负荷的反应。
DOI：
10.1159/000126431
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发表时间：
1993
影响因子：
4.1
通讯作者：
Morris,M
中科院分区：
文献类型：
--
作者：
Rocha,MJ;Callahan,MF;Sundberg,DK;Morris,M
神经解剖学和生理学证据表明，压力感受器影响下丘脑加压素(VP)和催产素(OT)神经元。我们评估了去窦弓神经(SAD)对盐负荷的分子和内分泌反应的影响。给假手术组或SAD组大鼠饮用2%氯化钠溶液72h。因去神经大鼠食盐摄入量低于对照组而限制盐水摄入量作为第二对照组。测定血浆VP、OTT、渗透压和垂体后叶多肽含量。用计算机定量原位杂交技术检测脑组织中VP和OTmRNA的表达。盐负荷使对照组和SAD组大鼠血浆VP和OT水平有同等程度的升高，但失神经动物的脑垂体后叶多肽耗竭程度更大。盐负荷使SAD大鼠垂体VP和OT显著降低，分别为69.8±8.4%和68.3±4.0%。在对照组中，仅在对照组中，VP含量和OT含量没有下降。去神经大鼠对盐负荷的反应也发生了改变。SAD盐水组下丘脑室旁核OT mRNA标记的面积和强度均显著增加。盐对照组大鼠SON增加，限盐组大鼠SON无变化。对于VP mRNA，注意到的唯一变化是盐负荷的SAD动物的子代。这些结果表明，动脉压力感受器的慢性失神经增加了下丘脑VP和OT对盐负荷的反应。这反映在脑垂体肽的更大消耗和下丘脑信息水平的变化上。结果表明，失神经动物对盐刺激高度敏感，导致中枢OT和VP轴的激活。
Neuroanatomical and physiological evidence indicates that baroreceptors influence hypothalamic vasopressin (VP) and oxytocin (OT) neurons. We evaluated the effects of sinoaortic denervation (SAD) on the molecular and endocrine response to salt loading. Sham-operated or SAD rats were given a 2% NaCl solution to drink for 72 h. A group with limited salt water intake was included as a second control because the denervated rats consumed less salt than the controls. Plasma VP, OTand osmolality and posterior pituitary peptide content were measured. Brains were processed for evaluation of VP and OTmRNA expression using in situ hybridization with computer quantitation. Salt loading produced equivalent increases in plasma VP and OT in the control and SAD groups, however, there was a greater depletion of posterior pituitary peptides in the denervated animals. Salt loading produced significant decreases in pituitary VP and OT in the SAD animals, 69.8 ± 8.4% and 68.3 ± 4.0%, respectively. In the control groups, there was no decrease in VP content and a decrease in OT only in the control ad lib group. The peptide mRNA response to salt loading was also altered in the denervated rats. There was a significant increase in the area and intensity of the labeling for OT mRNA in the PVN in the SAD salt group. The control salt rats showed an increase in the SON and the salt-limited group showed no changes. For VP mRNA, the only change noted was in the SON of the salt-loaded SAD animals. These results show that chronic denervation of arterial baroreceptors augments the hypothalamic VP and OT response to salt loading. This is reflected in a greater depletion of pituitary peptides and by changes in hypothalamic message levels. The results suggest that the denervated animal is hypersensitive to salt stimuli, resulting in a greater activation of the central OT and VP axis.