c-Myc G-quadruplex is sensitively and specifically recognized by a fluorescent probe.
c-Myc G-quadruplex is sensitively and specifically recognized by a fluorescent probe.
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DOI：
10.1016/j.talanta.2021.122125
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发表时间：
2021-05
期刊：
影响因子：
6.1
通讯作者：
Ranran Sun;Xiaomeng Guo;Dawei Yang;Yalin Tang;Jie Lu;Hongxia Sun
中科院分区：
文献类型：
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作者：
Ranran Sun;Xiaomeng Guo;Dawei Yang;Yalin Tang;Jie Lu;Hongxia Sun
c-myc基因序列形成的g -四重体结构因其在生物学上的重要生理功能和在纳米技术中的广泛应用而备受关注。到目前为止，能够识别c-myc g -四重体的高特异性和高灵敏度的探针仍然很少。本工作提出了一种菁染料荧光探针Cy-1，该探针在水溶液中几乎没有荧光，但在识别c-myc g -四重体时荧光增强1000倍以上。Cy-1也具有良好的特异性，可以选择性地从其他多种g -四重体和非g -四重体结构中识别c-myc的g -四重体。这些特性使Cy-1成为纳米技术或生物学中c-myc g -四重体识别的一个有前途的探针。
The G-quadruplex structure formed by the c-myc gene sequence has attracted much attention due to its important physiological function in biology and wide application in nanotechnology. So far, probes capable of recognition of c-myc G-quadruplex with both high specificity and sensitivity are still scarce. This work presented a cyanine dye fluorescent probe named Cy-1, which has almost no fluorescence in aqueous solution, but showing more than 1000-fold fluorescence enhancement for recognizing c-myc G-quadruplex. Cy-1 also has good specificity and can selectively recognize c-myc G-quadruplex from other a variety of G-quadruplex and non-G-quadruplex structures. These properties make Cy-1 a promising probe for c-myc G-quadruplex recognition in nanotechnology or biology.