ISOLATION AND CULTURE OF CHONDROCYTES FROM HUMAN ADULT ARTICULAR CARTILAGE
ISOLATION AND CULTURE OF CHONDROCYTES FROM HUMAN ADULT ARTICULAR CARTILAGE
复制标题
DOI：
10.1002/art.1780100309
复制
发表时间：
1967-01-01
影响因子：
--
通讯作者：
BONNER, WM
中科院分区：
文献类型：
--
作者：
MANNING, WK;BONNER, WM
通过用胶原酶消化然后进行轻微的机械破坏，可以从成人软骨中分离出活的软骨细胞。软骨细胞的单层培养物显示细胞去分化且无法合成基质。器官培养物中软骨细胞的聚集保留了其产生基质的能力。所描述的方法应该有助于研究正常和异常软骨的软骨细胞的功能。
Viable chondrocytes have been isolated from human adult cartilage by digestion with collagenase followed by mild mechanical disruption. Monolayer cultures of chondrocytes showed dedifferentiation of the cells with failure to synthesize matrix. Aggregations of chondrocytes in organ cultures retained their capacity to produce matrix. The methods described should prove helpful in studying functions of chondrocytes from normal and abnormal cartilage.