T-cell subset counting and the fight against AIDS: Reflections over a 20-year struggle
T-cell subset counting and the fight against AIDS: Reflections over a 20-year struggle
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DOI：
10.1002/cyto.10097
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发表时间：
2002-04-15
期刊：
影响因子：
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通讯作者：
Janossy, G
中科院分区：
文献类型：
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作者：
Mandy, F;Nicholson, J;Janossy, G
值此CD4 T细胞计数20周年之际，回顾一下T淋巴细胞亚群免疫分型技术的故事。随着时间的推移，免疫表型分析已发展成为精确、可靠但复杂且昂贵的技术，需要新鲜的血液样本。过去十年普遍适用于临床流式细胞术的门控技术依赖于白细胞迅速恶化的形态分散特征。本期专门讨论 CD4 T 细胞计数的特刊介绍了大多数可用的新选项，这些选项将对 21 世纪第一个十年内如何实施该技术产生重大影响。在一系列原创出版物中，包括新的 NIH T 细胞子集计数指南，使用免疫逻辑参数的当代门控方案被作为更可靠、更实惠的免疫表型替代方案的一部分提出。这里提出的一些改进包括测定成本以及监测实验室间和实验室内性能的能力。显然，要有效打击人类免疫缺陷病毒（HIV）疫情，就必须覆盖资源匮乏的地区。降低检测成本，同时提高可靠性和耐用性是朝着正确方向迈出的一步。细胞计数法 (Clin. Cytometry) 50: 39-45, 2002。(C) 2002 Wiley-Liss, Inc.
The story of T-lymphocyte subset immunophenotyping technology is reviewed on the occasion of the 20th anniversary of CD4 T-cell enumeration. Overtime, immunophenotyping has evolved into precise, reliable, but complicated and expensive technology requiring fresh blood samples. The gating technologies that were universally adapted for clinical flow cytometry for the past decade relied on rapidly deteriorating morphological scatter characteristics of leukocytes. This special issue dedicated to CD4 T-cell enumeration features most of the available new options that will have a significant impact on how this technology will be implemented within the first decade of the 21st century. In a series of original publications, including the new NIH guideline for T-cell subset enumeration, contemporary gating protocols that use immunologically logical parameters are presented as part of the more reliable and affordable immunophenotyping alternative. Some of the improvements addressed here include the costs of the assays and the capacity to monitor interlaboratory and intralaboratory performances. It is clear that an effective attack on the human immunodeficiency virus (HIV) epidemic has to embrace resource-poor regions. Reducing the cost of the assay while improving reliability and durability is a move in the right direction. Cytometry (Clin. Cytometry) 50: 39-45, 2002. (C) 2002 Wiley-Liss, Inc.