Light-up fluorescent probes utilizing binding behavior of perylenediimide derivatives to a hydrophobic pocket within DNA
Light-up fluorescent probes utilizing binding behavior of perylenediimide derivatives to a hydrophobic pocket within DNA
复制标题
利用苝二酰亚胺衍生物与 DNA 疏水性口袋的结合行为发光荧光探针
作者：
Takada;T.; Yamaguchi;K.; Tsukamoto;S.; Nakamura;M.; Yamana;K.
在这里，我们研究了？研究了含烷氧基的苝酰亚胺衍生物对电子转移猝灭的抑制作用。由于聚集诱导的淬灭，PO随机结合到DNA显示可忽略的荧光，而PO作为单体形式结合到口袋显示超过100倍的荧光增强。切换PO的结合状态对应于杂交事件的荧光响应的变化，这使我们能够设计具有纳摩尔检测限的核酸荧光传感器。
Here we study the binding behavior of perylenediimide (PDI) derivatives to a hydrophobic pocket created inside DNA and their photochemical properties capable of designing a light-up fluorescent sensor for short single-stranded DNA or RNA. The perylenediimide derivative with alkoxy groups (PO) suppressing electron transfer quenching was examined. The PO bound randomly to DNA showed negligible fluorescence due to the aggregation-induced quenching, whereas the PO bound to the pocket as a monomeric form showed more than 100-fold fluorescence enhancement. Switching the binding states of the PO corresponded to a change in the fluorescence response for the hybridization event, which allowed us to design a fluorescent sensor of nucleic acids with a nanomolar detection limit.