microRNA-146a downregulates IL-17 and IL-35 and inhibits proliferation of human periodontal ligament stem cells
microRNA-146a downregulates IL-17 and IL-35 and inhibits proliferation of human periodontal ligament stem cells
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DOI：
10.1002/jcb.28659
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发表时间：
2019-08-01
影响因子：
4
通讯作者：
Kim, Jong Ghee
中科院分区：
文献类型：
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作者：
Zhao, Sen;Cheng, Yawei;Kim, Jong Ghee
牙周炎的特点是促炎因子水平升高，例如白细胞介素 17 (IL-17) 和 IL-35。本研究分别采用定量逆转录聚合酶链反应和酶联免疫吸附试验检测牙周炎患者和健康对照血浆中微小RNA-146a（miRNA-146a）、IL-17和IL-35的表达。 miRNA-146a 模拟物被转染到从受牙周炎影响的牙齿和健康牙齿中分离的牙周膜干细胞 (PDLSC) 中。分别采用细胞计数试剂盒8法和Western blot分析检测细胞增殖情况以及IL-17和IL-35的表达。研究发现，与健康对照相比，牙周炎患者血浆中 miRNA-146a 表达下调，但 IL-17 和 IL-35 表达上调。牙周炎患者中 miRNA-146a 与 IL-17 和 IL-35 呈负相关。 miRNA-146a 过度表达抑制来自受牙周炎影响的牙齿和健康牙齿的 PDLSC 的增殖。 miRNA-146a 过度表达导致从受牙周炎影响的牙齿分离的 PDLSC 中 IL-17 和 IL-35 表达下调。因此，我们得出结论，miRNA-146a 可能通过下调 IL-17 和 IL-35 表达并抑制人 PDLSC 增殖来改善牙周炎。
Periodontitis is characterized by increased levels of proinflammatory factors, such as interleukin-17 (IL-17) and IL-35. In this study, the expression of microRNA-146a (miRNA-146a), IL-17, and IL-35 in the plasma of patients with periodontitis and healthy controls were detected by quantitative reverse transcription polymerase chain reaction and enzyme-linked immunosorbent assay, respectively. miRNA-146a mimic was transfected into periodontal ligament stem cells (PDLSCs) isolated from periodontitis-affected teeth and healthy teeth. Cell proliferation and expression of IL-17 and IL-35 were detected by cell counting kit-8 assay and Western blot analysis, respectively. It was observed that miRNA-146a was downregulated but IL-17 and IL-35 were upregulated in the plasma of patients with periodontitis than in healthy controls. miRNA-146a was inversely correlated with IL-17 and IL-35 in patients with periodontitis. miRNA-146a overexpression inhibited proliferation of PDLSCs derived from both periodontitis-affected teeth and healthy teeth. miRNA-146a overexpression led to downregulated IL-17 and IL-35 expression in PDLSCs isolated from periodontitis-affected teeth. We, therefore, conclude that miRNA-146a may improve periodontitis by downregulating IL-17 and IL-35 expression and inhibiting proliferation of human PDLSCs.