HETEROGENEITY OF ENDOTHELIAL-CELLS OF ADULT-RAT LIVER AS RESOLVED BY SEDIMENTATION-VELOCITY AND FLOW-CYTOMETRY
HETEROGENEITY OF ENDOTHELIAL-CELLS OF ADULT-RAT LIVER AS RESOLVED BY SEDIMENTATION-VELOCITY AND FLOW-CYTOMETRY
复制标题
DOI：
10.1002/jcp.1041190311
复制
发表时间：
1984-01-01
影响因子：
5.6
通讯作者：
LAFLEUR, L
中科院分区：
文献类型：
--
作者：
MORIN, O;PATRY, P;LAFLEUR, L
从成年大鼠肝脏分离的窦状细胞通过1 × 10 - 4的速度沉降分级。g（主要基于大小），并通过流式细胞术基于前向和垂直光散射和自发荧光进一步分析各种细胞级分。还使用Coulter计数器测量细胞体积。至少4个富集的细胞群在速度沉降后被分辨。它们分别对应于模态直径为6.5、7.5、9和11 μ m的细胞。各种细胞群的透射电子显微镜（TEM）分析揭示7.5 μ m和9 μ m细胞部分代表2种不同类型的内皮细胞，而11 μ m细胞对应于库普弗细胞。6.5 μ m的细胞被鉴定为淋巴细胞。通过其自发荧光和脂质含量鉴定的脂肪储存细胞包括在库普弗群体中。透射电镜显示，较小的内皮细胞具有数量和相对较少的回缩筛板比大的。这种超微结构特征可能与肝腺泡内2类内皮细胞的差异定位有关。
Sinusoidal cells isolated from adult rat liver were fractionated by velocity sedimentation at 1 .times. g (primarily on the basis of size) and the various cell fractions were further analyzed by flow cytometry on the basis of forward and perpendicular light scattering and autofluorescence. Cell volume was also measured using a Coulter counter. At least 4 enriched cell populations were resolved after velocity sedimentation. They corresponded to cells having a modal diameter of 6.5, 7.5, 9, and 11 .mu.m, respectively. Transmission electron microscopy (TEM) analysis of the various cell populations revealed that the 7.5- and 9-.mu.m cell fractions represented 2 distinct classes of endothelial cells while the 11-.mu.m cells corresponded to Kupffer cells. The 6.5-.mu.m cells were identified as lymphocytes. Fat-storing cells, identified by their autofluorescence and lipid content, were included in the Kupffer population. TEM demonstrated that the smaller endothelial cells possessed quantitatively and relatively less retracted sieve plates than the larger ones. This ultrastructural feature can be possibly correlated to a differential localization of the 2 classes of endothelial cells within the liver acinus.