The novel 2016 WHO Neisseria gonorrhoeae reference strains for global quality assurance of laboratory investigations: phenotypic, genetic and reference genome characterization
The novel 2016 WHO Neisseria gonorrhoeae reference strains for global quality assurance of laboratory investigations: phenotypic, genetic and reference genome characterization
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DOI：
10.1093/jac/dkw288
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发表时间：
2016-11-01
影响因子：
5.2
通讯作者：
Harris, Simon R.
中科院分区：
文献类型：
--
作者：
Unemo, Magnus;Golparian, Daniel;Harris, Simon R.
淋病和耐多药淋病奈瑟菌仍然是全球公共卫生问题。在全世界范围内加强有质量保证的淋球菌抗菌素耐药性（AMR）监测至关重要。世卫组织全球淋球菌抗菌素监测方案于2009年重新启动。我们描述了2016年WHO淋球菌参考菌株的表型、遗传和参考基因组特征，这些参考菌株旨在用于WHO全球GASP、其他GASP、全球诊断和研究的质量保证。2016年WHO参考菌株（n=14）构成了8种2008年WHO参考菌株和6种新菌株。新的菌株代表了低水平到高水平的头孢菌素耐药性，高水平的阿奇霉素耐药性和porA突变体。对23株耐药菌株进行了耐药谱、血清型、前亚氨基肽酶、质粒型、耐药性分子决定簇、耐药菌株、耐药耐药菌株、耐药菌株、耐药耐药淋病多抗原序列分型ST和MLST ST。使用单分子PacBio测序产生完整的参考基因组。参考菌株代表了所有可用的表型，敏感和耐药，以前和目前使用或考虑将来用于淋病治疗的抗菌药物。描述了相应的耐药基因型和分子流行病学类型。2016年世卫组织淋球菌参考菌株旨在用于实验室研究中的内部和外部质量保证和质量控制，特别是在世卫组织全球GASP和其他GASP中，但也用于表型（例如培养、种属确定）和分子诊断、分子AMR检测、分子流行病学和充分表征，在WGS分析、转录组学、蛋白质组学和其他分子技术和数据分析中注释和完成参考基因组。
Gonorrhoea and MDR Neisseria gonorrhoeae remain public health concerns globally. Enhanced, quality-assured, gonococcal antimicrobial resistance (AMR) surveillance is essential worldwide. The WHO global Gonococcal Antimicrobial Surveillance Programme (GASP) was relaunched in 2009. We describe the phenotypic, genetic and reference genome characteristics of the 2016 WHO gonococcal reference strains intended for quality assurance in the WHO global GASP, other GASPs, diagnostics and research worldwide.The 2016 WHO reference strains (n=14) constitute the eight 2008 WHO reference strains and six novel strains. The novel strains represent low-level to high-level cephalosporin resistance, high-level azithromycin resistance and a porA mutant. All strains were comprehensively characterized for antibiogram (n=23), serovar, prolyliminopeptidase, plasmid types, molecular AMR determinants, N. gonorrhoeae multiantigen sequence typing STs and MLST STs. Complete reference genomes were produced using single-molecule PacBio sequencing.The reference strains represented all available phenotypes, susceptible and resistant, to antimicrobials previously and currently used or considered for future use in gonorrhoea treatment. All corresponding resistance genotypes and molecular epidemiological types were described. Fully characterized, annotated and finished references genomes (n=14) were presented.The 2016 WHO gonococcal reference strains are intended for internal and external quality assurance and quality control in laboratory investigations, particularly in the WHO global GASP and other GASPs, but also in phenotypic (e.g. culture, species determination) and molecular diagnostics, molecular AMR detection, molecular epidemiology and as fully characterized, annotated and finished reference genomes in WGS analysis, transcriptomics, proteomics and other molecular technologies and data analysis.