PLASMA DEGRADATION OF PLATELET-ACTIVATING-FACTOR IN SEVERELY ILL PATIENTS WITH CLINICAL SEPSIS
PLASMA DEGRADATION OF PLATELET-ACTIVATING-FACTOR IN SEVERELY ILL PATIENTS WITH CLINICAL SEPSIS
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DOI：
10.1097/00003246-199402000-00009
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发表时间：
1994-02-01
影响因子：
8.8
通讯作者：
TAYLOR, RR
中科院分区：
文献类型：
--
作者：
GRAHAM, RM;STEPHENS, CJ;TAYLOR, RR
目的：研究临床脓毒症重症患者血浆血小板活化因子的降解情况。设计：前瞻性、非随机对照研究。地点：一所大学医院的重症监护室。患者：研究了13名因内科或外科疾病而患有临床败血症的危重男性患者和10名正常男性志愿者。在7名存活的患者中重复测量。测量和主要结果：乙酰水解酶的血浆活性，脂蛋白相关酶，水解血小板活化因子，其生物活性裂解衍生物的测定使用优化的酶试验。还测量了血小板活化因子的血浆半衰期，沿着磷脂酶Pq、活性、溶血血小板活化因子和血清脂质浓度。患者的结果与正常对照组的结果进行比较，并在幸存者中随访一次。患者组的乙酰水解酶活性显著低于正常受试者（中位数34，四分位数范围17至54 nmol/min/mL vs.中位数60，四分位数范围56至80 nmol/ min/mL;p
Objective: To study the plasma degradation of platelet-activating factor in severely ill patients with clinical sepsis. Design: A prospective, nonrandomized control study. Setting: Intensive care unit in a university hospital. Patients: Thirteen critically ill male patients with clinical sepsis, due to medical or surgical illness, and ten normal male volunteers were studied. Measurements were repeated in seven patients who survived. Measurements and Main Results: The plasma activity of acetylhydrolase, the lipoprotein-associated enzyme that hydrolyses platelet-activating factor to its biologically inactive lyse-derivative was determined using an optimized enzyme assay. The plasma half-life of platelet-activating factor was also measured, along with phospholipase Pq, activity, lyso-platelet-activating factor, and serum lipid concentrations. Patient results were compared with those results of normal controls and followed once in survivors. Acetylhydrolase activity in the patient group was significantly lower than in normal subjects (median 34, interquartile range 17 to 54 nmol/min/mL vs. median 60, interquartile range 56 to 80 nmol/ min/mL;p