The possible roles of mineralocorticoid receptor and 11β-hydroxy steroid dehydrogenase type 2 in cardiac fibrosis in the spontaneously hypertensive rat
The possible roles of mineralocorticoid receptor and 11β-hydroxy steroid dehydrogenase type 2 in cardiac fibrosis in the spontaneously hypertensive rat
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DOI：
10.1016/s0960-0760(03)00198-5
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发表时间：
2003-06-01
影响因子：
4.1
通讯作者：
Sasano, H
中科院分区：
文献类型：
--
作者：
Konishi, A;Tazawa, C;Sasano, H
在高血压中，醛固酮已被证实在心肌纤维化中起关键作用，而心肌纤维化通常会增加心脏疾病的发病率和死亡率。然而，很少有研究报道在高血压情况下心脏中盐皮质激素受体（MR）和11β - 羟基类固醇脱氢酶2型（11β - HSD2）的表达情况。因此，在本研究中，对自发性高血压大鼠（SHR）进行了研究，以阐明盐皮质激素通过与MR结合可能产生的作用。使用了京都威斯特大鼠（WKY）、SHR、易卒中SHR（SHRSP）和恶性SHRSP（M - SHRSP）。从这些大鼠的左心室提取总RNA，并使用Light Cycler仪器通过逆转录实时定量聚合酶链反应检测MR、11β - HSD2以及1型和3型胶原蛋白的表达水平。各组之间的血压存在显著差异。与WKY相比，M - SHRSP中MR、11β - HSD2以及1型和3型胶原蛋白的平均mRNA水平显著升高，而在SHR和SHRSP之间未检测到mRNA水平的显著差异。本研究的结果似乎表明，MR和11β - HSD2的mRNA在M - SHRSP的左心室中显著升高，并且这些mRNA的升高是心肌纤维化的原因之一。（C）2003爱思唯尔有限公司。保留所有权利。
In hypertension, aldosterone has been demonstrated to play a crucial role in cardiac fibrosis, which generally increases cardiac morbidity and death. However, few studies have reported the expression of the mineralocorticoid receptor (MR) and 11beta-hydroxysteroid dehydrogenase type 2 (11beta-HSD2) in the heart under hypertensive conditions. Therefore, in this study, spontaneously hypertensive rats (SHR) were examined to elucidate the possible actions of mineralocorticoids via binding to MR. Wister Kyoto Rat (WKY), SHR, stroke-prone SHR (SHRSP), and malignant SHRSP (M-SHRSP) were used. Total RNA was extracted from the left ventricle of these rats, and examined for the expression levels of MR, 11beta-HSD2 and Collagen types 1 and 3 using reverse transcription real-time quantitative polymerase chain reaction employing the Light Cycler Instrument. Blood pressure was significantly different among each group. The mean mRNA levels for MR, 11beta-HSD2 and Collagen types 1 and 3 in M-SHRSP were found to be significantly increased compared to those of WKY, whereas no significant differences in mRNA levels were detected among SHR and SHRSP. Findings from the present study appear to demonstrate that MR and 11beta-HSD2 mRNA significantly rise in the left ventricle of M-SHRSP and increase of these mRNA is one of the cause of cardiac fibrosis. (C) 2003 Elsevier Ltd. All rights reserved.