Evaluation of Ligand-Selector Interaction from Effective Diffusion Coefficient
Evaluation of Ligand-Selector Interaction from Effective Diffusion Coefficient
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DOI：
10.1021/ac1008207
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发表时间：
2010-07-01
影响因子：
7.4
通讯作者：
Holyst, Robert
中科院分区：
文献类型：
--
作者：
Bielejewska, Anna;Bylina, Andrzej;Holyst, Robert
我们提出了一种确定配体选择器平衡结合常数的分析方法。该方法是基于在有选择器和没有选择器的情况下，通过长（约25 m），薄（0.254 mm 0.05 +/- mm ID）毛细管的泊泽维尔流动时配体的有效分子扩散系数的测量。用泰勒色散理论对数据进行了分析。以牛血清白蛋白（BSA）和环糊精（CD）为模型选择剂。我们在以下选择配体络合物上测试了我们的方法：牛血清白蛋白与华法林、心得安、诺斯卡平、水杨酸和核黄素，环糊精与4-硝基苯酚。结果与文献数据和我们自己在经典色谱法中得到的结果一致。这种方法对不带电和带电的化合物同样有效。
We present an analytical technique for determination of ligand-selector equilibrium binding constants. The method is based on the measurements of effective molecular diffusion coefficient of the ligand during Poiseuille flow through a long (approximately 25 m), thin (0.254 mm 0.05 +/- mm ID) capillary with and without the selector. The data are analyzed using the Taylor dispersion theory. Bovine Serum Albumin (BSA) and cyclodextrin (CD) were taken as model selectors. We have tested our method on the following selector-ligand complexes: BSA with warfarin, propranolol, noscapine, salicylic acid, and riboflavin, and cyclodextrin with 4-nitrophenol. The results are in good agreement with data from the literature and with our own results obtained within classical chromatography. This method works equally well for uncharged and charged compounds.