High-resolution imaging and evaluation of spines in organotypic hippocampal slice cultures.
High-resolution imaging and evaluation of spines in organotypic hippocampal slice cultures.
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DOI：
10.1007/978-1-61779-536-7_24
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发表时间：
2012-01-01
期刊：
影响因子：
--
通讯作者：
Bakota, Lidia
中科院分区：
文献类型：
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作者：
Sundermann, Frederik;Golovyashkina, Nataliya;Bakota, Lidia
在许多类型的神经元中，树突棘是兴奋性神经元输入的场所。脊柱形状与突触接触的强度和成熟度有关。因此，脊柱形态的评价对于神经元发育的研究，对于确定学习和记忆的形态相关性，以及分析神经退行性变的机制都是相关的。在这里，我们描述了一种从转基因或非转基因小鼠制备的器官型海马片培养的体外模型中确定脊柱形态的方法。脊柱使用共聚焦高分辨率成像，并通过基于算法的分析进行评估。该方法可以半自动地确定海马区树突段中不同类型的脊椎密度和分类，以评估海马区内的连通性。
Dendritic spines act as sites of excitatory neuronal input in many types of neurons. Spine shape correlates with the strength and maturity of synaptic contacts. Thus, evaluation of spine morphology is relevant for studies on neuronal development, for determination of morphological correlates of learning and memory, and for analysis of mechanisms of neurodegeneration. Here, we describe a method to determine spine morphology in an ex vivo model of organotypic hippocampal slice cultures prepared from transgenic or non-transgenic mice. Spines are imaged using confocal high-resolution imaging and evaluated by algorithm-based analysis. The approach permits semiautomated determination of spine density and classification of different spine types in dendritic segments from hippocampal subregions to evaluate intrahippocampal connectivity.