Immunoassay for Capsular Antigen of Bacillus anthracis Enables Rapid Diagnosis in a Rabbit Model of Inhalational Anthrax
Immunoassay for Capsular Antigen of Bacillus anthracis Enables Rapid Diagnosis in a Rabbit Model of Inhalational Anthrax
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DOI：
10.1371/journal.pone.0126304
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发表时间：
2015-05-05
期刊：
影响因子：
3.7
通讯作者：
Kozel, Thomas R.
中科院分区：
文献类型：
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作者：
Gates-Hollingsworth, Marcellene A.;Perry, Mark R.;Kozel, Thomas R.
吸入性炭疽是一种严重的生物威胁。吸入性炭疽的有效抗生素治疗需要早期诊断;疾病进展得越远，治愈的可能性就越小。目前的诊断手段，如血液培养，需要几天的结果，并需要先进的实验室基础设施。另一种诊断方法是检测炭疽杆菌抗原，该抗原可进入血液，并可通过快速免疫测定法检测。本研究的目的是评价多聚-γ-D-谷氨酸（PGA）（B的荚膜抗原）的检测。炭疽，作为吸入性炭疽兔模型血培养的生物标志物替代物。血培养阳性的平均时间为26 +/- 5.7 h（平均值+/-标准差），而ELISA阳性的平均时间为22 +/- 4.2 h;与血培养相比，P = 0.005。建立了一种侧流免疫测定法，用于检测血浆中浓度低于1 ng PGA/ml的PGA。在兔模型中使用侧流免疫测定法检测PGA发现，抗原的检测略早于血培养变为阳性的最早时间点。侧流免疫测定形式的低成本、易用性和快速获得结果的时间使PGA免疫测定成为血培养的可行替代物，用于检测可能暴露于B的个体中的感染。炭疽病
Inhalational anthrax is a serious biothreat. Effective antibiotic treatment of inhalational anthrax requires early diagnosis; the further the disease has progressed, the less the likelihood for cure. Current means for diagnosis such as blood culture require several days to a result and require advanced laboratory infrastructure. An alternative approach to diagnosis is detection of a Bacillus anthracis antigen that is shed into blood and can be detected by rapid immunoassay. The goal of the study was to evaluate detection of poly-gamma-D-glutamic acid ( PGA), the capsular antigen of B. anthracis, as a biomarker surrogate for blood culture in a rabbit model of inhalational anthrax. The mean time to a positive blood culture was 26 +/- 5.7 h ( mean +/- standard deviation), whereas the mean time to a positive ELISA was 22 +/- 4.2 h; P = 0.005 in comparison with blood culture. A lateral flow immunoassay was constructed for detection of PGA in plasma at concentrations of less than 1 ng PGA/ml. Use of the lateral flow immunoassay for detection of PGA in the rabbit model found that antigen was detected somewhat earlier than the earliest time point at which the blood culture became positive. The low cost, ease of use, and rapid time to result of the lateral flow immunoassay format make an immunoassay for PGA a viable surrogate for blood culture for detection of infection in individuals who have a likelihood of exposure to B. anthracis.