Synthesis of nano-textured biocompatible scaffolds from chicken eggshells
Synthesis of nano-textured biocompatible scaffolds from chicken eggshells
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DOI：
10.1088/0957-4484/23/47/475601
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发表时间：
2012-11-30
期刊：
影响因子：
3.5
通讯作者：
Iqbal, Samir M.
中科院分区：
文献类型：
--
作者：
Asghar, Waseem;Kim, Young-Tae;Iqbal, Samir M.
细胞粘附、形态和生长受到纳米和微米尺度的表面形貌的影响。纳米纹理表面是使用光刻、等离子体蚀刻和长聚合物化学蚀刻来制备的，这些方法成本高昂且需要专门的设备。这篇文章展示了一种简单的方法来合成纳米纹理支架从鸡蛋壳。蛋壳上有各种各样的图案，如微针森林和纳米孔，使表面具有非常均匀的纳米纹理。表面的特征在于化学成分和晶相。新的图案被转移到PDMS表面和纳米纹理的PDMS表面被用来研究纹理对人成纤维细胞的生长和附着的影响。表面形貌的影响，沿着层粘连蛋白涂层细胞培养，也进行了研究。我们发现了一个令人兴奋的现象，即成纤维细胞的初始接种密度会影响纳米纹理对细胞生长的影响。与平坦的PDMS表面相比，这些纳米纹理表面提供16倍以上的成纤维细胞生长。新的纳米纹理图案还使层粘连蛋白在PDMS上的吸附加倍。
Cell adhesion, morphology and growth are influenced by surface topography at nano and micrometer scales. Nano-textured surfaces are prepared using photolithography, plasma etching and long polymer chemical etching which are cost prohibitive and require specialized equipment. This article demonstrates a simple approach to synthesize nano-textured scaffolds from chicken eggshells. Varieties of pattern are made on the eggshells like micro-needle forests and nanopores, giving very uniform nano-textures to the surfaces. The surfaces are characterized for chemical composition and crystal phase. The novel patterns are transferred to PDMS surfaces and the nano-textured PDMS surfaces are used to study the effect of texturing on human fibroblast cell growth and attachment. The effects of surface topographies, along with laminin coating on cell cultures, are also studied. We find an exciting phenomenon that the initial seeding density of the fibroblast cells affects the influence of the nano-texturing on cell growth. These nano-textured surfaces give 16 times more fibroblast growth when compared to flat PDMS surfaces. The novel nano-textured patterns also double the laminin adsorption on PDMS.