Diauxic Growth of Azotobacter vinelandii on Galactose and Glucose: Regulation of Glucose Transport by Another Hexose
Diauxic Growth of Azotobacter vinelandii on Galactose and Glucose: Regulation of Glucose Transport by Another Hexose
复制标题
维氏固氮菌在半乳糖和葡萄糖上的双生长：另一种己糖对葡萄糖转运的调节
DOI：
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发表时间：
1995
影响因子：
4.4
通讯作者：
G. Childers
中科院分区：
文献类型：
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作者：
T. Wong;H. Pei;K. Bancroft;G. Childers
当维氏固氮菌在含有半乳糖和葡萄糖的混合物的培养基中生长时，该生物体的生长曲线是双相的。半乳糖是主要碳源；葡萄糖也被消耗，但在生长的第一阶段消耗的速率低于半乳糖的消耗速率。两种糖的代谢途径均被诱导。当半乳糖耗尽时，细胞培养物表现出第二个滞后期。该滞后期的长度从 2 小时到 10 小时不等，具体取决于细胞的生长前历史。第二个对数生长期以培养基中剩余的葡萄糖为代价而发生，并伴随着葡萄糖诱导的葡萄糖通透酶的高最大代谢率的诱导以及葡萄糖代谢酶水平的增加。 diauxie 的第二个滞后期可能是由于诱导葡萄糖诱导的葡萄糖通透酶所需的时间所致。
The growth curve of Azotobacter vinelandii was biphasic when the organism was grown in a medium containing a mixture of galactose and glucose. Galactose was the primary carbon source; glucose was also consumed, but the rate at which it was consumed was lower than the rate at which galactose was consumed during the first phase of growth. Metabolic pathways for both sugars were induced. Cell cultures exhibited a second lag period as galactose was depleted. The length of this lag phase varied from 2 to 10 h depending on the pregrowth history of the cells. The second log growth phase occurred at the expense of the remaining glucose in the medium and was accompanied by induction of the high-maximum rate of metabolism glucose-induced glucose permease and increases in the levels of glucose metabolic enzymes. The second lag phase of diauxie may have been due to the time required for induction of the glucose-induced glucose permease.