Characterisation of chicken monocytes, macrophages and interdigitating cells by the monoclonal antibody KUL01
Characterisation of chicken monocytes, macrophages and interdigitating cells by the monoclonal antibody KUL01
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DOI：
10.1016/s0165-2427(97)00152-9
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发表时间：
1998-02-27
影响因子：
1.8
通讯作者：
Berghman, LR
中科院分区：
文献类型：
--
作者：
Mast, J;Goddeeris, BM;Berghman, LR
用单克隆抗体KUL 01对鸡单核吞噬细胞系统的分布、功能和个体发育进行了研究。KUL 01特异性识别鸡单核细胞，巨噬细胞和交错细胞，以及活化的小胶质细胞。其组织分布允许区分KUL 01从所有先前描述的单克隆抗体，单核吞噬细胞反应。在功能测定中进一步证实了KUL 01对单核吞噬细胞的特异性：脾脏和肝脏中的KUL 01阳性巨噬细胞主动摄取胶体碳，而单核细胞以及脾脏和肠道巨噬细胞含有抗原加工特征性的非特异性酯酶和酸性磷酸酶活性。此外，证明了KUL 01反应性外周血单核细胞表达MHC-II，但不表达CD 4。在所有的组织调查，相同的形态亚型的巨噬细胞检测在鸡在类似的定位在哺乳动物中，表明高度的保护之间的单核吞噬细胞系统的鸡和哺乳动物。(C)1998年Elsevier Science B.V.
The distribution, function and ontogeny of the mononuclear phagocyte system of the chicken were characterised using the monoclonal antibody (MAb) KUL01. KUL01 specifically recognises chicken monocytes, macrophages and interdigitating cells, as well as activated microglia cells. Its tissue distribution allowed to discriminate KUL01 from all earlier described MAb, reactive with mononuclear phagocytes. The specificity of KUL01 for mononuclear phagocytes was further confirmed in functional assays: KUL01-positive macrophages in spleen and liver actively took up colloidal carbon, while monocytes and spleen and gut macrophages contained non-specific esterase and acid phosphatase activities characteristic for antigen-processing. Further, it was demonstrated that KUL01-reactive peripheral blood monocytes express MHC-II, but not CD4. In all tissues investigated, the same morphological subtypes of macrophages were detected in chicken at similar localisations as in mammals, indicating a high degree of conservation between the mononuclear phagocyte system of the chicken and of mammals. (C) 1998 Elsevier Science B.V.