A phage satellite tunes inducing phage gene expression using a domesticated endonuclease to balance inhibition and virion hijacking.
A phage satellite tunes inducing phage gene expression using a domesticated endonuclease to balance inhibition and virion hijacking.
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DOI：
10.1093/nar/gkab207
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发表时间：
2021-05-07
影响因子：
14.9
通讯作者：
Seed KD
中科院分区：
文献类型：
--
作者：
Netter Z;Boyd CM;Silvas TV;Seed KD
细菌持续受到细菌病毒(噬菌体)捕食的威胁。细菌与噬菌体的冲突导致进化军备竞赛，通常是由移动遗传元件(MGES)驱动的。霍乱弧菌中的噬菌体卫星PLE就是一种这样的MGE，它对普遍存在的裂解噬菌体ICP1提供了特异而强大的防御。PLE和ICP1之间的相互作用揭示了分子寄生的策略，允许PLE劫持ICP1过程以进行动员。在这里，我们描述了PLE介导的ICP1结构基因转录调控的机制。PLE编码一种新的DNA结合蛋白CAPR，它抑制ICP1‘S衣壳形态发生操纵子。虽然CAPR足以满足PLE实现的衣壳抑制程度，但其活性并不妨碍ICP1的生命周期。我们探索了抑制这个操纵子的后果，证明了通过CRISPRi实现的更严格的抑制既限制了ICP1，也限制了PLE。我们还发现，PLE在修饰后的ICP1类颗粒中有转导作用。对CAPR同源物的检测导致了一套ICP1编码的归巢内切酶的鉴定，为卫星编码的抑制子提供了一个推测的来源。这项工作揭示了卫星介导的抑制的微妙平衡的一个方面，旨在阻止噬菌体的产生，同时成功地动员在噬菌体衍生的颗粒中。
Bacteria persist under constant threat of predation by bacterial viruses (phages). Bacteria-phage conflicts result in evolutionary arms races often driven by mobile genetic elements (MGEs). One such MGE, a phage satellite in Vibrio cholerae called PLE, provides specific and robust defense against a pervasive lytic phage, ICP1. The interplay between PLE and ICP1 has revealed strategies for molecular parasitism allowing PLE to hijack ICP1 processes in order to mobilize. Here, we describe the mechanism of PLE-mediated transcriptional manipulation of ICP1 structural gene transcription. PLE encodes a novel DNA binding protein, CapR, that represses ICP1’s capsid morphogenesis operon. Although CapR is sufficient for the degree of capsid repression achieved by PLE, its activity does not hinder the ICP1 lifecycle. We explore the consequences of repression of this operon, demonstrating that more stringent repression achieved through CRISPRi restricts both ICP1 and PLE. We also discover that PLE transduces in modified ICP1-like particles. Examination of CapR homologs led to the identification of a suite of ICP1-encoded homing endonucleases, providing a putative origin for the satellite-encoded repressor. This work unveils a facet of the delicate balance of satellite-mediated inhibition aimed at blocking phage production while successfully mobilizing in a phage-derived particle.
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影响因子：
14.9
作者：
Barth, Zachary K.;Silvas, Tania V.;Seed, Kimberley D.
通讯作者：
Seed, Kimberley D.
影响因子：
16.6
作者：
Pinilla-Redondo R;Shehreen S;Marino ND;Fagerlund RD;Brown CM;Sørensen SJ;Fineran PC;Bondy-Denomy J
通讯作者：
Bondy-Denomy J
影响因子：
4.5
作者：
O'Hara, Brendan J.;Barth, Zachary K.;Seed, Kimberley D.
通讯作者：
Seed, Kimberley D.
影响因子：
16.8
作者：
Edgell, DR;Derbyshire, V;Belfort, M
通讯作者：
Belfort, M
DOI：
10.1021/la504407v
发表时间：
2015
期刊：
Langmuir : the ACS journal of surfaces and colloids
影响因子：
--
作者：
Clifton LA;Skoda MW;Le Brun AP;Ciesielski F;Kuzmenko I;Holt SA;Lakey JH
通讯作者：
Lakey JH