Novel multiplex PCR assay for detection of the staphylococcal virulence marker panton-valentine leukocidin genes and simultaneous discrimination of methicillin-susceptible from -resistant staphylococci
Novel multiplex PCR assay for detection of the staphylococcal virulence marker panton-valentine leukocidin genes and simultaneous discrimination of methicillin-susceptible from -resistant staphylococci
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DOI：
10.1128/jcm.44.3.1141-1144.2006
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发表时间：
2006-03-01
影响因子：
9.4
通讯作者：
Zhang, KY
中科院分区：
文献类型：
--
作者：
McClure, JA;Conly, JM;Zhang, KY
我们建立了一种新的多重聚合酶链式反应方法，用于检测潘通-瓦伦丁杀白素毒力基因，并同时区分甲氧西林敏感和耐药葡萄球菌。该检测方法简单、快速、准确，为迅速检测新出现的社区耐甲氧西林金黄色葡萄球菌提供了可能。
We developed a new multiplex PCR assay for detection of Panton-Valentine leukocidin virulence genes and simultaneous discrimination of methicillin-susceptible from -resistant staphylococci. This assay is simple, rapid, and accurate and offers the potential for prompt detection of newly emerging community-associated methicillin-resistant Staphylococcus aureus.