Tetherin restricts herpes simplex virus 1 and is antagonized by glycoprotein M.
Tetherin restricts herpes simplex virus 1 and is antagonized by glycoprotein M.
复制标题
DOI：
10.1128/jvi.02250-13
复制
发表时间：
2013-12
影响因子：
5.4
通讯作者：
Towers GJ
中科院分区：
文献类型：
--
作者：
Blondeau C;Pelchen-Matthews A;Mlcochova P;Marsh M;Milne RS;Towers GJ
Tetherin是一种广泛有效的抗病毒效应物，其作用是将新生的包膜病毒粒子拴在宿主细胞膜上，从而阻止它们释放。在这项研究中，我们证明单纯疱疹病毒1 （HSV-1）是tetherin的靶标。我们鉴定出病毒包膜糖蛋白M （gM）具有中等抗tetherin活性。我们发现，转基因而不是gB或gD能有效地去除质膜上的系绳蛋白，并能在功能上替代人类免疫缺陷病毒1型（HIV-1） Vpu蛋白，即典型的病毒系绳蛋白拮抗剂，从表达系绳蛋白的细胞中拯救HIV-1释放。我们的数据强调，tetherin是一种广泛有效的抗病毒效应物，并有助于新出现的假设，即病毒必须抑制或逃避一系列宿主细胞对策，以建立生产感染。
Tetherin is a broadly active antiviral effector that works by tethering nascent enveloped virions to a host cell membrane, thus preventing their release. In this study, we demonstrate that herpes simplex virus 1 (HSV-1) is targeted by tetherin. We identify the viral envelope glycoprotein M (gM) as having moderate anti-tetherin activity. We show that gM but not gB or gD efficiently removes tetherin from the plasma membrane and can functionally substitute for the human immunodeficiency virus type 1 (HIV-1) Vpu protein, the prototypic viral tetherin antagonist, in rescuing HIV-1 release from tetherin-expressing cells. Our data emphasize that tetherin is a broadly active antiviral effector and contribute to the emerging hypothesis that viruses must suppress or evade an array of host cell countermeasures in order to establish a productive infection.