T cells with Fc receptors for IgA: induction of T alpha cells in vivo and in vitro by purified IgA.
T cells with Fc receptors for IgA: induction of T alpha cells in vivo and in vitro by purified IgA.
复制标题
具有 IgA Fc 受体的 T 细胞：通过纯化的 IgA 在体内和体外诱导 T α 细胞。
作者：
R. Hoover;B. Dieckgraefe;R. Lynch
先前的研究表明，患有 IgA 分泌性浆细胞瘤 MOPC-315（α lambda 2）、MOPC-167（α kappa）、McPC-603（α kappa）和 TEPC-15（α kappa）的 BALB/c 小鼠发育出大量具有 IgA 表面膜受体的 T 细胞（T α 细胞）。患有变异性浆细胞瘤（IgA 非分泌者或低分泌者）的小鼠中 T α 细胞扩增缺乏，这表明血清 IgA 升高导致 T α 细胞增加。目前的研究表明，每天注射 30 毫克 IgA（M315 蛋白）的正常 BALB/c 小鼠的 T α 细胞数量显着增加。这些研究还表明，注射IgA诱导的Tα细胞是Lyt1-2+ T细胞。此外，所提供的数据表明，在体外用纯化的聚合 IgA（M315 蛋白）处理尼龙毛非贴壁 T 细胞后，T α 细胞数量显着增加。 IgA 体外诱导 T α 细胞需要 DNA 和蛋白质合成。这些发现表明，在患有 IgA 骨髓瘤的小鼠中观察到的 T α 细胞扩增与 IgA 的高血清水平有关，而不是与骨髓瘤肿瘤本身有关。此外，这些观察结果与 B 细胞调节问题具有更普遍的相关性，因为它们证明分泌的免疫球蛋白可以直接诱导免疫调节 T 细胞的扩增。
Previous studies demonstrated that BALB/c mice with the IgA-secreting plasmacytomas MOPC-315 (alpha lambda 2), MOPC-167 (alpha kappa), McPC-603 (alpha kappa) and TEPC-15 (alpha kappa) developed large numbers of T cells with surface membrane receptors for IgA (T alpha cells). The lack of T alpha cell expansion in mice with variant plasmacytomas that were nonsecretors or low secretors of IgA implied that elevated serum IgA contributed to the increase in T alpha cells. The present studies show that normal BALB/c mice that were given daily injections of 30 mg of IgA (M315 protein) develop a marked increase in the number of T alpha cells. These studies also show that the T alpha cells induced by injection of IgA are Lyt1-2+ T cells. In addition, the data presented demonstrate that nylon wool nonadherent T cells, treated with purified polymeric IgA (M315 protein) in vitro, develop a marked increase in the number of T alpha cells. The in vitro induction of T alpha cells by IgA requires DNA and protein synthesis. These findings indicate that the T alpha cell expansion observed in mice with IgA myeloma is related to the high serum level of IgA and not to the myeloma tumor per se. In addition, these observations have a more general relevance to the issue of B cell regulation because they demonstrate that secreted immunoglobulin can directly induce expansion of immunoregulatory T cells.