Identification of two nerve growth factor-induced polypeptides in PC12 cells.
Identification of two nerve growth factor-induced polypeptides in PC12 cells.
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PC12 细胞中两种神经生长因子诱导的多肽的鉴定。
DOI：
10.1007/bf02896841
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
Fowler,VM
中科院分区：
文献类型：
--
作者：
Sussman,MA;Battenberg,E;Bloom,FE;Fowler,VM
我们已经在PC12细胞中鉴定出两种神经生长因子（NGF）诱导的多肽（Mr80，000和90，000），它们是热稳定的且未硫酸化。这些多肽在神经生长因子处理的PC12细胞的间接免疫荧光定位揭示了集中在神经突的点状图案。大鼠组织的免疫过氧化物酶染色表明，这些多肽被发现在整个大脑中的选定的神经元的子集，并在垂体或肾上腺髓质缺席。这些特征中的几个与最近描述的NGF诱导的分泌蛋白（VGF8a）相似，其与分泌颗粒蛋白具有序列相似性。这些多肽的NGF诱导特性和在大鼠组织中的分布都是分泌颗粒蛋白的新特征。
We have identified two nerve growth factor (NGF)-induced polypeptides (Mr80,000 and 90,000) in PC12 cells that are heat stable and not sulfated. Indirect immunofluorescence localization of these polypeptides in NGF-treated PC12 cells reveals a punctate pattern concentrated in neurites. Immunoperoxidase staining of rat tissue shows that these polypeptides are found throughout the brain in selected subsets of neurons and are absent in the pituitary or adrenal medulla. Several of these characteristics are similar to a recently described NGF-induced secretory protein (VGF8a), which has sequence similarities to secretogranins. The property of NGF inducibility and the distribution of these polypeptides within rat tissues are both novel features for secretogranin proteins.