Proteomic profile of carbonylated proteins in rat liver: Exercise attenuated oxidative stress may be involved in fatty liver improvement
Proteomic profile of carbonylated proteins in rat liver: Exercise attenuated oxidative stress may be involved in fatty liver improvement
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大鼠肝脏中羰基化蛋白质的蛋白质组学特征：运动减弱的氧化应激可能与脂肪肝的改善有关
DOI：
10.1002/pmic.201200522
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发表时间：
2013-05-01
期刊：
影响因子：
3.4
通讯作者：
Xie, Qingji
中科院分区：
文献类型：
--
作者：
Hu, Xiaofei;Duan, Zhigui;Xie, Qingji
为了筛选介导运动预防非酒精性脂肪性肝病（NAFLD）作用的氧化应激靶蛋白，改进了选择羰基化蛋白的方法，并对羰基化蛋白进行了谱分析。结果表明，跑台训练降低了氧化应激和肝内甘油三酯（IHTG）水平。IHTG的变化与丙二醛水平所指示的氧化应激呈显著正相关。蛋白质组学的进一步结果表明，17个功能蛋白质易受氧化修饰，运动保护三个蛋白质免受羰基化。后三种蛋白质可能既是氧化应激的直接靶蛋白，也是促进运动有益作用的介质。特别是，脂肪代谢关键酶长链特异性酰基辅酶A脱氢酶（ACADL）在运动组中没有被羰基化，活性更高。这些发现表明，这种改进的技术是实用的和强大的选择羰基化蛋白质。长期跑步机训练可有效改善氧化应激和防止IHTG的积累。在17种氧化修饰的靶蛋白中，有三种蛋白有助于运动的有益效果。防止ACADL羰基化可能参与运动诱导的NAFLD改善的生理机制。
To screen target proteins of oxidative stress which mediate the effects of exercise on preventing nonalcoholic fatty liver disease (NAFLD), the methods for selecting carbonylated proteins were modified, and carbonylated proteins were profiled. The results showed that treadmill training reduced oxidative stress and the levels of intrahepatic triglyceride (IHTG). The changes in IHTG showed a significant positive correlation with oxidative stress as indicated by malondialdehyde level. Further results from proteomics illustrated that 17 functional proteins were susceptible to oxidative modification, and exercise protected three proteins from carbonylation. The latter three proteins may serve as both direct target proteins of oxidative stress and mediators contributing to the beneficial effects of exercise. In particular, a long‐chain specific acyl‐CoA dehydrogenase (ACADL) which was a key enzyme in lipid metabolism was not carbonylated and with higher activities in exercise group. These findings indicate that this modified technique is practical and powerful in selecting carbonylated proteins. Long‐term treadmill training is effective in ameliorating oxidative stress and preventing the accumulation of IHTG. Among the 17 target proteins of oxidative modification, three proteins contribute to the beneficial effects of exercise. Preventing ACADL from carbonylation may be involved in the physiological mechanism of exercise‐induced NAFLD improvement.