Leukotriene generation by eosinophils.
Leukotriene generation by eosinophils.
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嗜酸性粒细胞产生白三烯。
DOI：
10.1084/jem.155.2.390
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发表时间：
1982
期刊：
影响因子：
--
通讯作者：
Klebanoff,SJ
中科院分区：
文献类型：
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作者：
Jörg,A;Henderson,WR;Murphy,RC;Klebanoff,SJ
当与钙离子载体A23187孵育时，纯化至98%以上的马嗜酸性粒细胞产生慢反应物质（SRS）。在每个细胞的基础上，嗜酸性粒细胞产生的SRS的四到五倍，由类似处理的马中性粒细胞产生。5，8，11，14-二十碳四炔酸可抑制嗜酸性粒细胞SRS的产生，吲哚美辛和花生四烯酸可增强嗜酸性粒细胞SRS的产生，提示嗜酸性粒细胞SRS是花生四烯酸代谢的脂氧合酶途径的产物。具有SRS活性的化合物通过高压液相色谱（HPLC）纯化，并通过紫外光谱、用脂氧合酶处理时的光谱位移、[14 C]花生四烯酸的掺入、气相色谱-质谱以及HPLC上的保留时间与真实标准的比较来鉴定。嗜酸性粒细胞产物的特征是5-（S），12-（R）-二羟基-6-顺式-8，10-反式-14-顺式-二十碳四烯酸（白三烯B4）及其5-（S），12-（R）-6-反式和5-（S），12-（S）-6-反式异构体，5-（S）-羟基-6-（R）-S-谷胱甘肽-7，9-反式-11，14-顺式-二十碳四烯酸（白三烯C4）及其11-反式异构体，和5-（S）-羟基-6-（R）-S-半胱氨酰甘氨酸-7，9-反式-11，14-顺式-二十碳四烯酸（白三烯D4）。
Horse eosinophils purified to greater than 98% generated slow reacting substance (SRS) when incubated with the calcium ionophore A23187. On a per cell basis, eosinophils generated four to five times the SRS produced by similarly treated horse neutrophils. Eosinophil SRS production was inhibited by 5,8,11,14-eicosatetraynoic acid and augmented by indomethacin and arachidonic acid, suggesting that it was a product(s) of the lipoxygenase pathway of arachidonic acid metabolism. Compounds with SRS activity were purified by high-pressure liquid chromatography (HPLC) and identified by ultraviolet spectra, spectral shift on treatment with lipoxygenase, incorporation of [14C]arachidonic acid, gas chromatography-mass spectrometry, and comparison of retention times on HPLC to authentic standards. The eosinophil products characterized were 5-(S), 12-(R)-dihydroxy-6-cis-8, 10-trans-14-cis-eicosatetraenoic acid (leukotriene B4) and its 5-(S), 12-(R)-6-trans and 5-(S), 12-(S)-6-trans isomers, 5-(S)-hydroxy-6-(R)-S-glutathionyl-7,9-trans-11, 14-cis-eicosatetraenoic acid (leukotriene C4) and its 11-trans isomer, and 5-(S)-hydroxy-6-(R)-S-cysteinylglycine-7,9-trans-11,14-cis-eicosatetraenoic acid (leukotriene D4).