Ku80 gene knockdown by the CRISPR/Cas9 technique affects the biological functions of human thyroid carcinoma cells
Ku80 gene knockdown by the CRISPR/Cas9 technique affects the biological functions of human thyroid carcinoma cells
复制标题
DOI：
10.3892/or.2019.7348
复制
发表时间：
2019-12-01
期刊：
影响因子：
4.2
通讯作者：
Wang, Juanhong
中科院分区：
文献类型：
--
作者：
Fan, Yali;Li, Jianying;Wang, Juanhong
为探讨Ku 80在甲状腺癌（TC）中的作用，采用免疫组织化学方法检测了86例甲状腺疾病患者甲状腺组织中Ku 80、核因子-κ B（NF-κ B）和RET/TC的蛋白水平。此外，在TC细胞中，通过逆转录-定量PCR分析检测Ku 80 mRNA，并使用成簇规则间隔短回文重复序列（CRISPR）/CRISPR相关蛋白9（Cas9）技术沉默Ku 80 mRNA，以评估其作用。使用抗体阵列来鉴定Ku 80相关的调控基因。肿瘤组织中Ku 80蛋白表达水平显著高于癌旁组织（P
In the present study, to evaluate the role of Ku80 in thyroid carcinoma (TC), 86 thyroid tissue samples from patients with a spectrum of thyroid disorders were examined for protein levels of Ku80, nuclear factor-kappa B (NF-kappa B) and RET/TC by immunohistochemistry. Furthermore, in TC cells, Ku80 mRNA was detected by reverse transcription-quantitative PCR analysis and silenced using the clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) technique to assess its role. An antibody array was used to identify Ku80-related regulatory genes. The protein levels of Ku80 in the TC tissues were significantly higher than those in non-neoplastic adjacent tissue samples (P