SCHEDULE OF SPERMATOGENESIS IN THE PULMONATE SNAIL HELIX-ASPERSA, WITH SPECIAL REFERENCE TO HISTONE TRANSITION
SCHEDULE OF SPERMATOGENESIS IN THE PULMONATE SNAIL HELIX-ASPERSA, WITH SPECIAL REFERENCE TO HISTONE TRANSITION
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DOI：
10.1083/jcb.7.3.515
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发表时间：
1960-01-01
期刊：
影响因子：
--
通讯作者：
HEW, HYC
中科院分区：
文献类型：
--
作者：
BLOCH, DP;HEW, HYC
精子发生的时间表是由减数分裂前DNA合成过程中氚标记的细胞通过连续的精子发生阶段所需的时间来确定的。从一个典型的体细胞组蛋白富含赖氨酸，富含精氨酸的组蛋白的过渡被证明发生在精子细胞阶段。在成熟的精子中观察到后期向鱼精蛋白的转变。这些变化的特征在于使用原位染色方法。向富含精氨酸的组蛋白的转变伴随着氚标记的精氨酸的掺入，因此反映了新蛋白质的合成。精氨酸和胸苷掺入的时间，和独立的DNA测量的比较表明，在有丝分裂前染色体复制的情况下，在精子细胞中的组蛋白合成是不伴随DNA合成。在最初的组蛋白变化过程中，细胞核内形成细丝，细丝聚集形成薄片。这种精细结构在精子成熟过程中丢失。
The schedule of spermatogenesis is determined from the times necessary for cells labeled with tritium thymidine during premeiotic DNA synthesis to pass through the successive spermatogenic stages. A transition from a typically somatic histone rich in lysine, to a histone rich in arginine is shown to occur during spermatid stages. A later shift to a protamine is observed in the maturing sperm. These changes are characterized by the use of in situ staining methods. The transition to arginine-rich histone is accompanied by incorporation of tritium-labeled arginine, hence reflects synthesis of new protein. Comparison of the timing of arginine and thymidine incorporation, and independent measurements of DNA, show that in contrast to the case of premitotic chromosome duplication, the histone synthesis in the spermatid is unaccompanied by DNA synthesis. During the initial histone change, fine filaments are formed within the nucleus, which aggregate to form lamellae. This fine structure is lost during maturation of the sperm.