Singlet oxygen photosensitisation by GFP mutants: oxygen accessibility to the chromophore
Singlet oxygen photosensitisation by GFP mutants: oxygen accessibility to the chromophore
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DOI：
10.1039/c0pp00125b
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发表时间：
2010-01-01
影响因子：
3.1
通讯作者：
Nonell, Santi
中科院分区：
文献类型：
--
作者：
Jimenez-Banzo, Ana;Ragas, Xavier;Nonell, Santi
为了合理开发基因编码的光敏剂，利用时间分辨近红外发光检测技术对一类绿色荧光蛋白（GFP）突变体的单线态氧产生进行了研究。分子氧对发色团的可及性似乎在GFP使单线态氧光敏化的能力中起主要作用，并且这可以通过引入特定突变（例如用体积较小的氨基酸替换His148）来调节。GFP也是良好的单线态氧猝灭剂，因此该领域的进一步发展还应寻求消除具有最高猝灭能力的那些氨基酸，特别是在蛋白质表面和发色团附近的那些氨基酸。
Aiming at the rational development of genetically-encoded photosensitisers, the production of singlet oxygen has been assessed for a number of class-2 Green Fluorescent Protein (GFP) mutants by means of time-resolved near-infrared luminescence detection. The accessibility of molecular oxygen to the chromophore seems to play a major role in the ability of GFPs to photosensitise singlet oxygen and this can be modulated by introducing specific mutations such as replacement of His148 by a less bulky amino acid. GFPs are also good singlet oxygen quenchers, hence further developments in this area should also seek to eliminate those amino acids with the highest quenching ability, particularly those at the protein surface and in the vicinity of the chromophore.