MIG1-DEPENDENT AND MIG1-INDEPENDENT GLUCOSE REGULATION OF MAL GENE-EXPRESSION IN SACCHAROMYCES-CEREVISIAE
MIG1-DEPENDENT AND MIG1-INDEPENDENT GLUCOSE REGULATION OF MAL GENE-EXPRESSION IN SACCHAROMYCES-CEREVISIAE
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DOI：
10.1007/bf00309785
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发表时间：
1995-08-01
期刊：
影响因子：
2.5
通讯作者：
MICHELS, CA
中科院分区：
文献类型：
--
作者：
HU, Z;NEHLIN, JO;MICHELS, CA
葡萄糖抑制是酿酒酵母调控碳源利用、线粒体生物发生糖异生等代谢途径的全球性调控系统。Mig1p是一种锌指类dna结合蛋白，是葡萄糖反应中调节GAL和SUC基因表达的转录抑制因子。该报告表明，Mig1蛋白可以抑制MAL61和MAL62结构基因的转录，也可以抑制编码mal -激活子的MAL63基因的转录。在所有三个MAL基因的上游均鉴定出Mig1p dna结合位点。在双向MAL61-MAL62启动子中发现的两个mig1p结合位点被证明在mig1p依赖性葡萄糖抑制中起作用。利用组成型MAL激活子等位基因的研究表明，葡萄糖对诱导物可用性的调节是葡萄糖抑制MAL基因表达的第二个主要因素；和是;甚至比依赖migp的抑制成分更强。此外，我们的研究结果还提示了葡萄糖调节MAL基因表达的其他次要机制的贡献。
Glucose repression is a global regulatory system in Saccharomyces cerevisiae controlling carbon-source utilization, mitochondrial biogenesis gluconeogenesis and other metabolic pathways. Mig1p, a zinc-finger class of DNA-binding protein, is a transcriptional repressor regulating GAL and SUC gene expression in response to glucose. This report demonstrates that Mig1 protein represses transcription of the MAL61 and MAL62 structural genes and also the MAL63 gene, which encodes the Mal-activator. Mig1p DNA-binding sites were identified upstream of all three MAL genes. Both of the Mig1p-binding sites found in the bidirectional MAL61-MAL62 promoter were shown to function in the Mig1p-dependent glucose repression. Studies using constitutive Mal-activator alleles suggest that glucose regulation of inducer availability is a second major contributing factor in glucose repression of MAL gene expression; and is; even stronger than thp Mig1p-dependent component of repression. Moreover, our results also suggest the contribution of other minor mechanisms in glucose regulation of MAL gene expression.