Inhibition of translation and modification of translation factors during apoptosis induced by the DNA-damaging agent MMS in sea urchin embryos
Inhibition of translation and modification of translation factors during apoptosis induced by the DNA-damaging agent MMS in sea urchin embryos
复制标题
DOI：
10.1016/j.yexcr.2007.12.014
复制
发表时间：
2008-03-10
影响因子：
3.7
通讯作者：
Morales, Julia
中科院分区：
文献类型：
--
作者：
Le Bouffant, Ronan;Boulben, Sandrine;Morales, Julia
研究了海胆卵和胚胎对DNA损伤剂甲基磺酸酯（MMS）的翻译控制。我们已经表明，在这份报告中，海胆胚胎暴露于MMS诱导蛋白质合成活性，翻译因子水平，完整性和翻译后修饰的剧烈影响。响应于DNA损伤剂MMS对胚胎的处理，蛋白质合成被抑制，独立于翻译抑制剂4 E-BP，并且与翻译因子eIF 2 α亚基的磷酸化相关。此外，低分子量形式的翻译起始因子eIF 4G被检测到与MMS诱导的细胞凋亡相关。我们认为，翻译因子的修饰在DNA损伤诱导的细胞凋亡过程中发生的蛋白质合成调节中起着重要作用。(C)2008年爱思唯尔公司All rights reserved.
Translational control was investigated in sea urchin eggs and embryos in response to the DNA-damaging agent methyl methanesulfonate (MMS). We have shown in this report that exposure of sea urchin embryos to MMS induces drastic effects on protein synthesis activity, and on translation factors level, integrity and post-translational modifications. In response to the treatment of embryos by the DNA-damaging agent MMS, protein synthesis is inhibited independently of the translation inhibitor 4E-BP and in correlation with phosphorylation of the translation factor eIF2 alpha subunit. Furthermore, a low molecular weight form of translation initiation factor eIF4G is detected correlatively with MMS-induced apoptosis. We propose that modifications of translation factors play an important role in protein synthesis modulation that occurs during DNA-damage induced apoptosis. (C) 2008 Elsevier Inc. All rights reserved.