A low-molecular-weight ascophyllan prepared from Ascophyllum nodosum: Optimization, analysis and biological activities.
A low-molecular-weight ascophyllan prepared from Ascophyllum nodosum: Optimization, analysis and biological activities.
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DOI：
10.1016/j.ijbiomac.2020.02.334
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发表时间：
2020-03
影响因子：
8.2
通讯作者：
Gang Yu;Yanhong Chen;Qingyun Bao;Zedong Jiang;Yanbing Zhu;H. Ni;Qingbiao Li;T. Oda
中科院分区：
文献类型：
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作者：
Gang Yu;Yanhong Chen;Qingyun Bao;Zedong Jiang;Yanbing Zhu;H. Ni;Qingbiao Li;T. Oda
本研究以海藻酸解酶（EC 4.2.2.3）水解的葡萄树酚为原料，采用超滤分离法制备低分子量糖片段（LMWAs-L）。LMWAs-L为均匀的糖组分，平均分子量为6.96 kDa。酶解工艺优化实验表明，制备LMWAs-L的最佳工艺参数为酶浓度0.02 U/mL，初始pH 6.8，酶解温度43℃。优化后，LMWAs-L的产率比未优化时提高了9.74%。有趣的是，LMWAs-L在体外对人皮肤成纤维细胞的增殖和迁移具有更强的促进作用，并且在相同质量浓度下，其抗菌活性比天然葡萄酚更强。本研究建立了一种简单、高效地从葡萄叶青素中制备具有有益生物活性的低分子量糖的方法。这是首次发现葡萄酚及其低分子量糖具有开发作为天然生物敷料和抗菌剂的潜力。
In this study, a low-molecular-weight saccharide fragment (LMWAs-L) was prepared from alginate lyase (EC 4.2.2.3) hydrolyzed ascophyllan by ultra-filtration separation method. LMWAs-L was a homogeneous saccharide fraction with an average molecular weight of 6.96 kDa. Enzymolysis process optimization experiments revealed that the optimum process parameters for preparing LMWAs-L were the enzyme concentration 0.02 U/mL, initial pH 6.8, and enzymolysis temperature 43 °C. After optimization, the yield of LMWAs-L was increased to 9.74% higher than that without optimization. Interestingly, LMWAs-L exhibited stronger enhancing activities on the proliferation and migration of human skin fibroblasts cellsin vitroand better antibacterial activities as compared to native ascophyllan at the same mass concentration. Our study establishes a simple way to prepare low-molecular-weight saccharide with beneficial bioactivities from ascophyllan efficiently. This is the first report to reveal that ascophyllan and its low-molecular-weight saccharide have the potentials to be developed as natural biological dressing and antibacterial agents.