Assay of an activity in the serum of patients with disorders of thrombopoiesis that stimulates formation of megakaryocytic colonies.
Assay of an activity in the serum of patients with disorders of thrombopoiesis that stimulates formation of megakaryocytic colonies.
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测定患有血小板生成障碍的患者血清中刺激巨核细胞集落形成的活性。
DOI：
10.1056/nejm198109033051001
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发表时间：
1981
期刊：
影响因子：
--
通讯作者：
Floyd,V
中科院分区：
文献类型：
--
作者：
Hoffman,R;Mazur,E;Bruno,E;Floyd,V
我们最近描述了一个人巨核细胞祖细胞或巨核细胞集落形成单位（CFU-M）的体外克隆检测系统。从定量血小板紊乱患者血清标本筛选能力改变体外巨核细胞集落形成。11例低核细胞性血小板减少症患者的血清显著增强了cfu - m衍生菌落的形成（200%至1840 %）。8例血小板减少症患者的血清和11例血小板增多症患者的血清均未改变集落形成。这种刺激活性被称为巨核细胞集落刺激活性（Meg-CSA）。巨核细胞菌落的形成数量与刺激血清的添加量成正比。Meg-CSA水平与骨髓巨核细胞数量呈负相关。在不同疾病状态下检测到的Meg-CSA水平的变化表明，这种干细胞调节剂产生的改变具有生理重要性。（中华医学杂志。1981;35:533 - 538）
We have recently described an in vitro clonal assay system for human megakaryocyte-progenitor cells or megakaryocytic colony-forming units (CFU-M). Serum specimens from patients with quantitative platelet disorders were screened for the capacity to alter in vitro megakaryocyte-colony formation. Serum from 11 patients with hypomegakaryocytic thrombocytopenia significantly enhanced the formation of CFU-M-derived colonies (200 to 1840 per cent). Neither serum from eight patients with thrombocytopenia and normal or increased numbers of marrow megakaryocytes nor serum from 11 patients with thrombocytosis altered colony formation. This stimulatory activity has been termed megakaryocytic-colony-stimulating activity (Meg-CSA). The number of megakaryocytic colonies formed was directly proportional to the quantity of stimulatory serum added. Meg-CSA levels appeared to be inversely related to marrow megakaryocyte numbers. The variations in Meg-CSA levels that were detected in different disease states suggest that alterations in the production of this stem-cell regulator have physiologic importance. (N Engl J Med. 1981; 305:533–8.)