Designing optogenetically controlled RNA for regulating biological systems.
Designing optogenetically controlled RNA for regulating biological systems.
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DOI：
10.1111/nyas.12660
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发表时间：
2015-09
影响因子：
5.2
通讯作者：
Jaffrey SR
中科院分区：
文献类型：
--
作者：
You M;Jaffrey SR
光响应蛋白已被用于光遗传学领域来控制细胞功能。然而，令人惊讶的是，通过光调节和改变 RNA 分子功能的类似方法仍然不发达。 RNA适体和RNA装置可以执行多种细胞内功能，是合成生物学的重要工具。本报告探讨了将光调节工程改造为细胞功能性 RNA 的挑战和潜在策略。我们讨论了使用现有的光调节蛋白和小分子来控制活细胞中 RNA 功能的方法。此外，还讨论了光调节 RNA 在合成生物学和研究内源表达 RNA 功能中的应用。
Light-responsive proteins have been used in the field of optogenetics to control cellular functions. However, surprisingly, analogous approaches to regulate and alter the functions of RNA molecules by light remain underdeveloped. RNA aptamers and RNA devices can perform diverse intracellular functions and are important tools in synthetic biology. This report explores the challenges of and potential strategies for engineering light regulation into functional RNAs in cells. We discuss approaches for using existing light-regulated proteins and small molecules to control RNA function in living cells. Additionally, applications of light-regulated RNAs for synthetic biology and for studying functions of endogenously expressed RNAs are discussed.