Effects of temperature on sporulation and latent period of Colletotrichum spp. infecting strawberry fruit
Effects of temperature on sporulation and latent period of Colletotrichum spp. infecting strawberry fruit
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DOI：
10.1094/pdis.1997.81.1.77
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发表时间：
1997-01-01
期刊：
影响因子：
4.5
通讯作者：
Wilson, LL
中科院分区：
文献类型：
--
作者：
King, WT;Madden, LV;Wilson, LL
温度对尖孢炭疽菌（Colletotrichum acutatum，C. gloeosporioides和C.草莓炭疽病的原因，在控制环境的研究，确定。用分生孢子悬浮液接种分离的未成熟果实，并在5、10、15、20、25、30和35 ℃的恒温下孵育长达36天。潜伏期（至第一次孢子形成的时间）取决于温度，范围从25摄氏度的2至3天到5摄氏度的6至17天。C. acutatum有一个较短的潜伏期比其他物种在5和10摄氏度;在较高的温度下，该物种的潜伏期是非常相似的。在产孢的前4天，每果分生孢子数的对数[log（Y）]与温度之间存在最适型关系，在15 ~ 30 ℃时观察到最大产孢量（一般每果10（6）~ 10（7）个分生孢子）。在15摄氏度及以上的温度下，孢子形成随时间增加。在5 ℃和10 ℃时，物种之间的差异最大，其中测试的C。acutatum分离物比其它种类每果实多产生10 - 100个分生孢子。多项式回归方程被成功地用于表示log（Y）作为温度和孵育时间的函数。随着时间的推移，孢子形成的增加速率是温度的函数，预测的最佳温度为22至26摄氏度。方程进行了验证，通过预测孢子形成的三个物种感染水果附着在受控环境室中生长的植物。尽管预测值往往略大于观察值，但平均误差[100（观察值-预测值）/观察值]仅为-0.7%（95%置信区间：-2.4至1.0%）。
Effects of temperature on sporulation of Colletotrichum acutatum, C. gloeosporioides, and C. fragariae, causes of anthracnose of strawberry, were determined in controlled-environment studies. Detached immature fruit were inoculated with a conidial suspension and incubated up to 36 days at constant temperatures of 5, 10, 15, 20, 25, 30, and 35 degrees C. Latent period (time to first sporulation) depended on temperature and ranged from 2 to 3 days at 25 degrees C to 6 to 17 days at 5 degrees C. C. acutatum had a shorter latent period than the other species at 5 and 10 degrees C; at higher temperatures, latent periods of the species were very similar. During the first 4 days of sporulation, there was an optimum-type relationship between the logarithm of conidia per fruit [log(Y)] and temperature, with maximum observed sporulation (generally 10(6) to 10(7) conidia per fruit) from 15 to 30 degrees C. Sporulation increased over time at temperatures of 15 degrees C and above. The greatest difference among the species was at 5 and 10 degrees C, where tested C. acutatum isolates produced from 10 to 100 more conidia per fruit than the other species. Polynomial regression equations were used successfully to represent log(Y) as a function of temperature and incubation time. The rate of increase in sporulation over time was a function of temperature, with a predicted optimum of 22 to 26 degrees C. Equations were validated by predicting sporulation of the three species infecting fruit attached to plants growing in controlled-environment chambers. Although the predictions tended to be slightly larger than observed, mean error [100(observed - predicted)/ observed] was only -0.7% (95% confidence interval: -2.4 to 1.0%).