Translation of mRNA from calf thymus in the wheat germ system: evidence for a precursor of thymosin alpha1.
Translation of mRNA from calf thymus in the wheat germ system: evidence for a precursor of thymosin alpha1.
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小麦胚芽系统中小牛胸腺 mRNA 的翻译：胸腺素 α1 前体的证据。
DOI：
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发表时间：
1978
影响因子：
11.1
通讯作者：
B. Horecker
中科院分区：
文献类型：
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作者：
M. Freire;O. Crivellaro;C. Isaacs;J. Moschera;B. Horecker
当在小麦胚芽系统中翻译时，来自新鲜小牛胸腺的mRNA刺激放射性氨基酸掺入酸不溶性产物中，掺入的总放射性的10- 20%被活性胸腺素部分的抗血清沉淀。在十二烷基硫酸钠圆盘凝胶电泳中，主要在两种肽中回收到放射性，对应于16，000和11，000道尔顿;后者可能代表不完整的链。这些肽的胰蛋白酶解酶均产生相应于胸腺素α 1残基15- 19序列的片段;这些肽通过与合成胸腺素α 1的胰蛋白酶解酶共层析和Edman降解进行表征。因此，在无细胞系统中合成的16，000-道尔顿肽似乎是胸腺素α 1的q前体，并且可能是从小牛胸腺分离的级分中的其它肽的q前体。结果支持该肽在胸腺中合成的结论。
When translated in the wheat germ system, mRNA from fresh calf thymus stimulates incorporation of radioactive amino acids into an acid-insoluble product, and 10--20% of the total radioactivity incorporated is precipitated with antisera to active thymosin fractions. In sodium dodecyl sulfate disc gel electrophoresis, radioactivity was recovered mainly in two peptides, corresponding to 16,000 and 11,000 daltons; the latter probably represents incomplete chains. Tryptic digests of each of these peptides yielded fragments corresponding to the sequence of residues 15--19 of thymosin alpha1; these peptides were characterized by cochromatography with digests of synthetic thymosin alpha1 and by Edman degradation. Thus, the 16,000-dalton peptide synthesized in the cell-free system appears to be q precursor of thymosin alpha1 and possibly of other peptides in the fractions isolated from calf thymus. The results support the conclusion that this peptide is synthesized in the thymus gland.