NMR studies of nucleic acid dynamics.
NMR studies of nucleic acid dynamics.
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DOI：
10.1016/j.jmr.2013.08.014
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发表时间：
2013-12
影响因子：
2.2
通讯作者：
Al-Hashimi, Hashim M.
中科院分区：
文献类型：
--
作者：
Al-Hashimi, Hashim M.
核酸结构必须满足两个截然相反的要求；一方面，它们必须采用可以被蛋白质特异性识别的明确的3D结构；另一方面，它们的结构必须足够灵活，以便在关键的生化过程（包括复制、转录和翻译）中进行非常大的构象变化。核酸如何在不失去生物特异性的情况下将灵活性引入其3D结构？在这里，我描述了我的实验室中核磁共振光谱技术的发展和应用，用于表征核酸的动态特性，这些特性紧密结合了广泛的核磁共振测量，包括残余偶极耦合、自旋弛豫和弛豫色散，以及样品工程和计算方法。这种方法使我们对核酸的定向灵活性获得了基本的新见解，使它们的结构能够以非常特定的功能方式改变。
Nucleic acid structures have to satisfy two diametrically opposite requirements; on one hand they have to adopt well-defined 3D structures that can be specifically recognized by proteins; on the other hand, their structures must be sufficiently flexible to undergo very large conformational changes that are required during key biochemical processes, including replication, transcription, and translation. How do nucleic acids introduce flexibility into their 3D structure without losing biological specificity? Here, I describe the development and application of NMR spectroscopic techniques in my laboratory for characterizing the dynamic properties of nucleic acids that tightly integrate a broad set of NMR measurements, including residual dipolar couplings, spin relaxation, and relaxation dispersion with sample engineering and computational approaches. This approach allowed us to obtain fundamental new insights into directional flexibility in nucleic acids that enable their structures to change in a very specific functional manner.
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影响因子：
2.2
作者：
Al-Hashimi, HM;Valafar, H;Prestegard, JH
通讯作者：
Prestegard, JH
DOI：
10.1073/pnas.052675499
发表时间：
2002-03-05
影响因子：
11.1
作者：
Abrescia, NGA;Thompson, A;Subirana, JA
通讯作者：
Subirana, JA
影响因子：
15
作者：
Al-Hashimi, HM;Tolman, JR;Patel, DJ
通讯作者：
Patel, DJ
影响因子：
2.9
作者：
Bondensgaard, K;Mollova, ET;Pardi, A
通讯作者：
Pardi, A
影响因子：
14.9
作者：
Bardaro, Michael F., Jr.;Shajani, Zahra;Varani, Gabriele
通讯作者：
Varani, Gabriele