Fluorescence Anisotropy Studies on Bodipy (Pyrromethene 546) Dye as a Novel Thermal Probe
Fluorescence Anisotropy Studies on Bodipy (Pyrromethene 546) Dye as a Novel Thermal Probe
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DOI：
10.1007/s10895-021-02868-0
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发表时间：
2022-01
影响因子：
2.7
通讯作者：
P. Jain;M. Motosuke
中科院分区：
文献类型：
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作者：
P. Jain;M. Motosuke
在本工作中，BODIPY（pyrromethene 546或C14 H17 BF 2N 2）染料的荧光各向异性的研究已经进行，并发现它有可能被用作热探针测量微流体的温度。众所周知，染料在其激发态下旋转，因此为了控制染料在激发态下的分子旋转，在溶液中使用山梨糖醇。研究发现，山梨醇的加入使荧光各向异性增强，荧光寿命缩短。研究发现，加入山梨醇后，温度敏感性增加。为了研究-CH 2基团对荧光性质的影响，还使用了另一种BODIPY（吡咯甲烯597或C22 H33 BF 2 N2）染料，因为C14 H17 BF 2 N2和C22 H33 BF 2 N2具有相似的结构，仅-8CH 2基团不同。已经发现，C22 H33 BF 2 N2具有优于C14 H17 BF 2 N2的上级性质，即，与吡咯亚甲基546相比，吡咯亚甲基597显示出更大的温度敏感性，即使没有添加山梨糖醇。
In the present work, fluorescence anisotropy studies of BODIPY (pyrromethene 546 or C14H17BF2N2) dye have been performed and found that it has a potential to be used as a thermal probe to measure the temperature of microfluid. It is well-known that a dye rotates in its excited state, so to control the molecular rotation of the dye in the excited state, sorbitol is used in the solution. It has been found that adding sorbitol, fluorescence anisotropy increases, fluorescence lifetime decreases. It has been found that adding sorbitol, temperature sensitivity increases. To study the effect of -CH2groups on fluorescence properties, another BODIPY (pyrromethene 597 or C22H33BF2N2) dye is also used, as C14H17BF2N2and C22H33BF2N2have similar structures with a difference of only -8CH2groups. It has been found that C22H33BF2N2has superior properties over C14H17BF2N2, i.e., pyrromethene 597 shows larger temperature sensitivity as compared to pyrromethene 546, even without the addition of sorbitol.