Glycosilated nucleolin as marker for human gliomas
Glycosilated nucleolin as marker for human gliomas
复制标题
DOI：
10.1002/jcb.23381
复制
发表时间：
2012-02-01
影响因子：
4
通讯作者：
Cimini, A.
中科院分区：
文献类型：
--
作者：
Galzio, R.;Rosati, F.;Cimini, A.
核苷是一种多功能DNA和RNA结合蛋白，参与了基因转录，染色质重塑，RNA代谢和核糖体RNA合成的调节。核仁素似乎在高度增殖的细胞中过表达，并且参与了基因表达的许多方面：DNA重组和复制，RNA聚合酶I和II的RNA转录，RRNA加工，mRNA稳定，细胞因子和凋亡。尽管核苷主要位于核仁中，但也已证明它位于磷酸化/甘油的形式中，在不同细胞的细胞表面上。最近已经发表了许多用于涉及表面核苷靶向肿瘤疗法的文章。但是，目前，迄今为止，尚无广泛的信息可用于人神经胶质瘤中的核苷。在目前的工作中，我们调查了核仁蛋白在神经胶质瘤在不同等级的恶性肿瘤和通过手术切除得出的原发性神经胶质瘤细胞培养物上的存在和定位，试图将糖丝膜的膜核素与恶性等级相关联。为此，已经使用了美国对GP273蛋白产生的抗体，该抗体已被证明已识别糖胶状表面核醇蛋白。获得的结果表明，表面核仁素随着恶性等级的增加而增加，因此表明它可能构成用于神经胶质瘤分级的组织病理学标记，并且可能是靶向治疗的可能的工具。 J. Cell。生物化学。 113：571579，2012。（c）2011年Wiley Werdionals，Inc。
Nucleolin is a multifunctional DNA and RNA binding protein involved in regulation of gene transcription, chromatin remodeling, RNA metabolism, and ribosomal RNA synthesis. Nucleolin seems to be over-expressed in highly proliferative cells and is involved in many aspect of gene expression: DNA recombination and replication, RNA transcription by RNA polymerase I and II, rRNA processing, mRNA stabilization, cytokinesis, and apoptosis. Although nucleolin is localized predominantly in the nucleolus, it has also been shown to be localized in a phosphorylated/glycolsilated form on the cell surface of different cells. Numerous articles dealing with surface nucleolin targeting for tumor therapy have been recently published. However, at present, no extensive informations are so far available for the presence of nucleolin in human gliomas. In the present work we investigated on the presence and localization of nucleolin in glioma on glioma specimens at different grade of malignancy and on primary glioma cell cultures derived by surgical resection, trying to correlate the presence of glycosilated membrane nucleolin with the malignancy grade. To this purpose an antibody produced by us against gp273 protein, demonstrated to recognized the glycosilated surface nucleolin, has been used. The results obtained demonstrate that surface nucleolin increase with the malignancy grade thus suggesting that it may constitute a histopathological marker for glioma grading and a possible tool for targeted therapy. J. Cell. Biochem. 113: 571579, 2012. (C) 2011 Wiley Periodicals, Inc.