Aberrant microRNA expression in human cervical carcinomas
Aberrant microRNA expression in human cervical carcinomas
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DOI：
10.1007/s12032-011-9830-2
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发表时间：
2012-06-01
期刊：
影响因子：
3.4
通讯作者：
Lin, Zhongqiu
中科院分区：
文献类型：
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作者：
Rao, Qunxian;Zhou, Hui;Lin, Zhongqiu
由于在多种病变组织中已经观察到microRNAs （miRNAs）表达模式的改变，因此本研究比较了人类宫颈癌组织中miRNA相对于邻近正常宫颈组织的表达。采用924个探针的微阵列芯片检测了13例宫颈癌患者（宫颈鳞癌11例、宫颈腺癌1例、宫颈肉瘤1例）宫颈癌组织和邻近正常宫颈组织中miRNAs的表达，这些患者均感染了人乳头瘤病毒（HPV） 16和/或HPV18。与正常宫颈组织的表达水平相比，宫颈癌组织中有18个mirna（1.9%）表达水平显著上调（增加1‰），19个mirna（2.1%）表达水平显著下调（降低1‰）。miRNA的表达与淋巴结受累、血管浸润和病理分化无关。综上所述，宫颈癌组织相对于邻近正常组织的mirna表达发生了改变。miRNA的异常表达是否与宫颈癌的发病有关，还需进一步研究。
Because altered microRNAs (miRNAs) expression patterns have been observed in a variety of diseased tissues, miRNA expression was compared in human cervical cancer tissues relative to adjacent normal cervical tissues in the present study. Microarray chips with 924 probes were used to detect the expression of miRNAs in cervical cancer tissue and adjacent normal cervical tissue of 13 patients with cervical cancer (11 squamous cervical cancers, one cervical adenocarcinoma, and one cervical sarcoma), all of whom were infected with human papilloma virus (HPV) 16 and/or HPV18. Compared to the expression levels in normal cervical tissues, 18 miRNAs (1.9%) were significantly upregulated (increase of a parts per thousand yen2x), and 19 miRNAs (2.1%) were significantly downregulated (decrease of a parts per thousand currency sign0.5x) in cervical cancer tissues. miRNA expression was independent of lymph node involvement, vascular invasion, and pathological differentiation. Taken together, cervical cancer tissues have altered expression of miRNAs relative to adjacent normal tissues. Further studies are necessary to determine whether aberrant miRNA expression is related to the pathogenesis of cervical cancer.