Effect of Thiolated Glycosaminoglycans on the Behavior of Breast Cancer Cells: Toward the Development of in vitro Models of Cancer
Effect of Thiolated Glycosaminoglycans on the Behavior of Breast Cancer Cells: Toward the Development of in vitro Models of Cancer
复制标题
硫醇化糖胺聚糖对乳腺癌细胞行为的影响：促进体外癌症模型的开发
DOI：
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发表时间：
2017
影响因子：
1.7
通讯作者：
T. Groth
中科院分区：
文献类型：
--
作者：
Alexander Köwitsch;Ankur Chhalotre;T. Groth
细胞外基质成分如糖胺聚糖（glycosaminoglycans， GAG）或黏附蛋白对癌细胞迁移和肿瘤发生进展的影响仍然是一个具有挑战性的任务。因此，本研究旨在深入了解具有不同转移潜力的癌细胞（MDA-MB-231, MDA-MB-468）与表面固定化GAG与血清蛋白如纤连蛋白的相互作用。方法将不同硫代GAG（透明质酸（HA）、硫酸软骨素（CS）、肝素（Hep））共价包覆在模型基质上，用表面等离子体共振研究纤维连接蛋白（FN）的吸附。然后，研究了在血清蛋白存在和不存在的情况下乳腺癌细胞的粘附性。此外，在细胞迁移室和单细胞迁移的帮助下，用延时显微镜检查了融合癌细胞的生长行为。结果FN吸附结果表明，hep包被表面吸附的蛋白质明显多于CS和HA。一般来说，与无血清条件下相比，两种细胞系在HA-和cs -包被的血清蛋白存在的情况下，乳腺癌细胞在gag包被基质上的初始粘附受到抑制。血清蛋白对细胞大小的影响也明显减小。体外生长研究清楚地证实了两种癌细胞的迁移速度不同，而单细胞迁移在ha涂层表面上尤其增强。结论血清蛋白（如白蛋白）的吸附对乳腺癌细胞的粘附有抑制作用，两种乳腺癌细胞系对HA的单细胞迁移均有增强作用。
Purpose The influence of extracellular matrix components like glycosaminoglycans (GAG) or adhesive proteins on the migration of cancer cells and the progression of tumorigenesis remains a challenging task. Therefore, this study aims to give insight into the interaction of cancer cells exhibiting different metastatic potential (MDA-MB-231, MDA-MB-468) with surface immobilized GAG interacting with serum proteins like fibronectin. Methods Model substrata were covalently coated with different thiolated GAG (hyaluronan (HA), chondroitin sulfate (CS), heparin (Hep)) and investigated for the adsorption of fibronectin (FN) with surface plasmon resonance. Then, adhesion of breast cancer cells in the presence of and without serum proteins was studied. Further, the outgrow behavior of confluent cancer cells was examined with the help of cell migration chambers and single-cell migration with time-lapse microscopy. Results FN adsorption revealed that the Hep-coated surfaces were able to adsorb significantly more protein than CS and HA. Generally, initial adhesion of breast cancer cells on GAG-coated substrata was inhibited for HA- and CS-coated substrata in the presence of serum proteins for both cell lines in comparison to serum-free conditions. The cell size was also significantly decreased by the influence of serum proteins. The outgrow studies clearly confirmed the different migration speed of both cancer cells while single-cell migration was particularly enhanced on HA-coated surfaces. Conclusions The results reveal that adsorption of serum proteins (e.g. albumin) possess an inhibiting effect on the adhesion of breast cancer cells and that single-cell migration is enhanced for both breast cancer cell lines on HA.
影响因子：
6.9
作者：
Casu, Benito;Naggi, Annamaria;Torri, Giangiacomo
通讯作者：
Torri, Giangiacomo
DOI：
10.1016/s0021-9258(17)41727-3
发表时间：
1994-02
期刊：
The Journal of biological chemistry
影响因子：
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作者：
F. Barkalow;J. Schwarzbauer
通讯作者：
F. Barkalow;J. Schwarzbauer
影响因子：
3.3
作者：
Weyers, Amanda;Yang, Bo;Linhardt, Robert J.
通讯作者：
Linhardt, Robert J.