Promotion and Inhibition of the Oxidase‐Mimicking Activity of Nanoceria by Phosphate, Polyphosphate, and DNA
Promotion and Inhibition of the Oxidase‐Mimicking Activity of Nanoceria by Phosphate, Polyphosphate, and DNA
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DOI：
10.1002/cbic.202000049
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发表时间：
2020-03
期刊：
影响因子：
3.2
通讯作者：
Yilin Zhao;Haotian Li;Anand Lopez;Haijia Su;Juewen Liu
中科院分区：
文献类型：
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作者：
Yilin Zhao;Haotian Li;Anand Lopez;Haijia Su;Juewen Liu
纳米氧化铈（CeO2纳米颗粒）是一种被广泛研究的纳米酶，具有有趣的氧化酶模拟活性。由于CeO2纳米颗粒可以在没有有毒和不稳定的H2O2的情况下工作，因此已被广泛用于生物传感。CeO2纳米颗粒经常遇到含磷酸盐的分子，这可能会影响其催化活性，文献中存在各种报告，显示促进和抑制活性。在这项工作中，我们系统地研究了五种类型的磷酸盐：正磷酸盐，焦磷酸盐，三磷酸盐，三偏磷酸盐和具有25个磷酸盐单元的多磷酸盐（Pi25）。此外，各种长度和序列的DNA寡核苷酸。包括DNA，因为它们含有可以强烈吸附在纳米氧化铈上的磷酸盐主链。我们观察到，乙酸盐缓冲液中高浓度的DNA抑制活性，而磷酸盐缓冲液中低浓度的DNA增加活性。活性的变化还与底物的种类有关，并与CeO2的聚集有关。这些发现为CeO2纳米颗粒在生物传感器开发、材料科学和纳米技术中的进一步应用提供了重要的认识。
Nanoceria (CeO2 nanoparticles) is an extensively studied nanozyme with interesting oxidase‐mimicking activity. As they can work in the absence of toxic and unstable H2O2, CeO2 nanoparticles have been widely used in biosensing. CeO2 nanoparticles often encounter phosphate‐containing molecules that can affect their catalytic activity, and various reports exist in the literature showing both promoted and inhibited activity. In this work, we systematically studied five types of phosphate: orthophosphate, pyrophosphate, triphosphate, trimetaphosphate, and a polyphosphate with 25 phosphate units (Pi25). In addition, DNA oligonucleotides of various length and sequence. DNA was included as they contain a phosphate backbone that can strongly adsorb on nanoceria. We observed that a high concentration of DNA in acetate buffer inhibited activity, whereas a low concentration of DNA in phosphate buffer increased activity. The change of activity was also related to the type of substrate and related to the aggregation of CeO2. These discoveries provide an important understanding for the further use of CeO2 nanoparticles in biosensor development, materials science, and nanotechnology.