Serologic and Molecular Studies of Leptospira and Leptospirosis among Rats in the Philippines
Serologic and Molecular Studies of Leptospira and Leptospirosis among Rats in the Philippines
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DOI：
10.4269/ajtmh.2010.09-0711
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发表时间：
2010-05-01
影响因子：
3.3
通讯作者：
Yoshida, Shin-ichi
中科院分区：
文献类型：
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作者：
Villanueva, Sharon Y. A. M.;Ezoe, Hirokazu;Yoshida, Shin-ichi
已知大鼠是钩端螺旋体病最重要的宿主和传播源。然而。菲律宾钩端螺旋体病在水库和传播方面的状况仍然未知。在马尼拉大都会和拉古纳进行了一项调查，分析了106只老鼠的样本。显微镜凝集试验发现，92%的大鼠血清样品抗钩端螺旋体抗体阳性；最常见的感染血清型是马尼拉病毒。Hebdomadis和Losbanos。通过脉冲场凝胶电泳和gyrase B基因序列分析，分离出4组大鼠肾分离物：马尼拉乳杆菌、洛斯巴诺乳杆菌、伤寒乳杆菌和Javanica博格peterseni乳杆菌。大多数分离株在实验感染金色叙利亚仓鼠后致死。结果表明，这4种钩端螺旋体血清型和血清群在大鼠中流行，这些动物可能是菲律宾钩端螺旋体病的可能传播源之一。
Rats are known to be the most important reservoirs and transmission sources of leptospirosis. However. the status of leptospirosis in the Philippines regarding reservoirs and transmission remains unknown. A survey was conducted in Metro Manila and Laguna that analyzed samples obtained from 106 rats. Using the microscopic agglutination test, we found that 92% of rat serum samples were positive for anti-Leptospira antibodies; the most common infecting serovars were Manilae. Hebdomadis, and Losbanos. On the basis of pulsed-field gel electrophoresis and gyrase B gene sequence analyses, four groups of rat kidney isolates were found: L. interrogans serovar Manilae, serovar Losbanos, and serogroup Grippotyphosa, and L. borgpetersenii serogroup Javanica. Most isolates were lethal after experimental infection of golden Syrian hamsters. Results showed that these four Leptospira serovars and serogroups are circulating among rats, and that these animals may be one of the possible transmission sources of leptospirosis in the Philippines.