Puerarin inhibits tetrodotoxin-resistant sodium current in rat dorsal root ganglion neurons.
Puerarin inhibits tetrodotoxin-resistant sodium current in rat dorsal root ganglion neurons.
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发表时间：
1996-03
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通讯作者：
H. Ji;T. Y. Wang
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作者：
H. Ji;T. Y. Wang
目的观察葛根素（Pue）对背根神经节（DRG）神经元慢钠电流的影响。方法采用全细胞膜片钳技术，记录年轻成年大鼠DRG神经元抗河豚毒素钠电流。结果：0.01 ~ 2 mmol。L-1对TTXr钠电流的抑制作用为9.5% ~ 83.2%。抑制作用是浓度依赖和部分可逆的，但不依赖于使用或电压。Pue不影响失活，但将半最大电导电位从-26 mV改变为-16 mV，表明激活过程受到抑制。结论Pue适度抑制大鼠DRG神经元TTXr钠电流。
AIM To test if puerarin (Pue) affects slow sodium current in dorsal root ganglion (DRG) neurons. METHODS Tetrodotoxin resistant (TTXr) sodium current was recorded with whole cell patch clamp technique on DRG neurons of young adult rats. RESULTS Pue 0.01-2 mmol.L-1 inhibited TTXr sodium current by 9.5%-83.2%. The inhibition was concentration-dependent and partially reversible, but was not use-dependent nor voltage-dependent. Pue did not affect the inactivation but changed the potential for half maximal conductance from -26 mV to -16 mV, suggesting the activation process was inhibited. CONCLUSION Pue moderately inhibits TTXr sodium current of rat DRG neurons.