Degradation of normal mRNA in the nucleus of Saccharomyces cerevisiae
Degradation of normal mRNA in the nucleus of Saccharomyces cerevisiae
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DOI：
10.1128/mcb.23.16.5502-5515.2003
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发表时间：
2003-08-01
影响因子：
5.3
通讯作者：
Sherman, F
中科院分区：
文献类型：
--
作者：
Das, B;Butler, JS;Sherman, F
通过对酿酒酵母nup 116-Delta菌株mRNA周转率的分析，鉴定了核mRNA降解（DRN）系统，该菌株在限制性温度下缺乏输出所有RNA（包括poly（A）mRNA）的能力。北方印迹、原位杂交和在nup 116-Delta菌株中用硫代巴比妥阻断转录揭示了细胞核中mRNA的快速降解，其被rrp 6-Delta、rail-Delta和cbc 1-Delta缺失抑制，但不被upfl-Delta缺失抑制，这表明DRN需要Rrp 6 p（一种3 '至5'的核外切核酸酶）、Rat 1 p（一种5 '至3'的核外切核酸酶）和Cbc 1 p，CBC的一种成分，核帽结合复合物，可将mRNA引导至降解位点。我们认为，保留在细胞核中的某些正常mRNA被DRN系统降解，类似于某些突变体中3'端形成缺陷的转录本的降解。
A nuclear mRNA degradation (DRN) system was identified from analysis of mRNA turnover rates in nup116-Delta strains of Saccharomyces cerevisiae lacking the ability to export all RNAs, including poly(A) mRNAs, at the restrictive temperature. Northern blotting, in situ hybridization, and blocking transcription with thiolutin in nup116-Delta strains revealed a rapid degradation of mRNAs in the nucleus that was suppressed by the rrp6-Delta, rail-Delta, and cbc1-Delta deletions, but not by the upfl-Delta deletion, suggesting that DRN requires Rrp6p, a 3'-to-5' nuclear exonuclease, the Rat1p, a 5'-to-3' nuclear exonuclease, and Cbc1p, a component of CBC, the nuclear cap binding complex, which may direct the mRNAs to the site of degradation. We propose that certain normal mRNAs retained in the nucleus are degraded by the DRN system, similar to degradation of transcripts with 3' end formation defects in certain mutants.