Porphyromonas gingivalis biofilms persist after chlorhexidine treatment
Porphyromonas gingivalis biofilms persist after chlorhexidine treatment
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DOI：
10.1111/eos.12050
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发表时间：
2013-06-01
影响因子：
1.9
通讯作者：
Ebisu, Shigeyuki
中科院分区：
文献类型：
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作者：
Yamaguchi, Mikiyo;Noiri, Yuichiro;Ebisu, Shigeyuki
洗必泰(CHX)葡萄糖酸盐有效地降低了生物被膜形成细菌的生存能力，如牙龈卟啉单胞菌。然而，完全去除生物膜是不可能的。本研究的目的是评估CHX葡萄糖酸盐处理后残留的牙龈假单胞菌生物膜在体外的潜在致病性。扫描和透射电子显微镜和共聚焦激光成像显示，用CHX葡萄糖酸盐处理可以破坏牙龈假单胞菌的生物膜形成细胞，但不会破坏生物膜。残留生物膜中蛋白质和碳水化合物的体积与对照组无显著差异。残留生物膜对超声波的物理抵抗力显著高于对照组。牙龈假单胞菌在残留生物膜上的黏附量高于唾液覆盖孔。这些发现表明，尽管CHX葡萄糖酸盐导致生物膜形成细胞的破坏，但来自破坏细胞的成分仍保留在生物膜中，这维持了它们的外部结构。此外，残留的生物膜可以作为形成新生物膜的支架。
Chlorhexidine (CHX) gluconate effectively reduces the viability of biofilm-forming bacteria, such as Porphyromonas gingivalis. However, it is impossible to completely remove biofilms. The goal of the present study was to assess the potential pathogenicity of residual P.gingivalis biofilms in vitro after treatment with CHX gluconate. Scanning and transmission electron microscopy and confocal laser imaging revealed that treatment with CHX gluconate disrupted individual biofilm-forming P.gingivalis cells but did not destroy the biofilms. The volumes of the protein and carbohydrate constituents in the residual biofilms were not significantly different from those of the controls. The physical resistance of the residual biofilms to ultrasonication was significantly higher than that of controls. The volume of P.gingivalis adherent to the residual biofilms was higher than that to saliva-coated wells. These findings suggest that although CHX gluconate caused disruption of biofilm-forming cells, the constituents derived from disrupted cells were maintained in the biofilms, which sustained their external structures. Moreover, the residual biofilms could serve as a scaffold for the formation of new biofilms.