Effects of exercise training on adipogenesis of stromal-vascular fraction cells in rat epididymal white adipose tissue
Effects of exercise training on adipogenesis of stromal-vascular fraction cells in rat epididymal white adipose tissue
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运动训练对大鼠附睾白色脂肪组织基质血管成分细胞脂肪生成的影响
DOI：
10.1111/j.1748-1708.2010.02159.x
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发表时间：
2010
期刊：
影响因子：
--
通讯作者：
H.
中科院分区：
文献类型：
--
作者：
Sakurai;T.;Endo;S.;Hatano;D.;Ogasawara;J.;Kizaki;T.;Oh-ishi;S.;Izawa;T.;Ishida;H. & Ohno;H.
目的：以前的研究表明，运动训练减少了白色脂肪组织（WAT）的质量相比，久坐不动的控制，较小的质量包含较少的脂肪细胞。然而，运动训练对脂肪形成的影响并不完全清楚。因此，我们重新检查了运动训练对WAT中脂肪细胞数量的影响，并且如果发现这种影响，则测试含有脂肪组织来源的干细胞（ADSC）的基质血管部分（SVF）细胞在来自运动训练（TR）大鼠的附睾WAT中的成脂反应。方法：将Wistar雄性大鼠分为两组：对照（C）和TR。TR大鼠在跑步机上进行9周的运动。 通过离心从附睾WAT分离含有ADSC的SVF细胞。结果：TR大鼠SVF细胞中过氧化物酶体增殖物激活受体γ（peroxisome proliferator‐activated receptor γ，PPARγ）及其靶基因的表达显著下调，前脂肪细胞因子-1基因的表达显著上调。与C组大鼠相比，TR组大鼠SVF细胞在诱导脂肪细胞分化后的脂质积累明显受到抑制。此外，在TR大鼠的SVF细胞中观察到缺氧诱导因子-1 α（HIF-1α）蛋白表达增加。结论：运动训练抑制了SVF细胞向脂肪细胞分化的能力，其机制与HIF-1α表达上调有关。
Aim:Previous studies have shown that exercise training reduced white adipose tissue (WAT) mass compared to that in sedentary controls, and that the smaller mass contained fewer adipocytes. However, the effect of exercise training on adipogenesis is not completely clear. Therefore, we re‐examined the effect of exercise training on adipocyte numbers in WAT and, if such an effect was found tested the adipogenic responses of stromal‐vascular fraction (SVF) cells containing adipose tissue‐derived stem cells (ADSC) in epididymal WAT from exercise‐trained (TR) rats.Methods:Wistar male rats were divided into two groups: control (C) and TR. The TR rats were subjected to exercise on a treadmill for 9 weeks. SVF cells containing ADSC were separated from epididymal WAT by centrifugation. Expression of adipocyte differentiation‐related genes and adipogenesis of SVF cells were examined.Results:In SVF cells of TR rats, the expression of peroxisome proliferator‐activated receptor γ (PPARγ) and that of PPARγ target lipogenic genes was dramatically downregulated, whereas that of preadipocyte factor‐1 gene was significantly upregulated. Lipid accumulation in SVF cells of TR rats after the induction of adipocyte differentiation was significantly suppressed in comparison with that of C rats. Moreover, increased expression of hypoxia‐inducible factor‐1α (HIF‐1α) protein was observed in SVF cells of TR rats. Pre‐treatment of YC‐1, a potent HIF‐1α inhibitor, in SVF cells of TR rats restored adipogenesis.Conclusion:These results suggest that exercise training suppresses the ability of SVF cells to differentiate into adipocytes, and that underlying mechanisms involve the upregulation of HIF‐1α expression.