Multiple promoters and targeted microRNAs direct the expressions of HMGB3 gene transcripts in dairy cattle
Multiple promoters and targeted microRNAs direct the expressions of HMGB3 gene transcripts in dairy cattle
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多个启动子和靶向 microRNA 指导奶牛 HMGB3 基因转录本的表达
DOI：
10.1111/age.12007
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发表时间：
2013-06-01
期刊：
影响因子：
2.4
通讯作者：
Zhong, Jifeng
中科院分区：
文献类型：
--
作者：
Li, Liming;Huang, Jinming;Zhong, Jifeng
HMGB 3（高迁移率族蛋白3）是一个X连锁的非序列依赖性染色质结合蛋白家族成员，在核酸介导的先天免疫应答中起着普遍的哨兵作用。研究牛HMGB 3基因的剪接变体表达、启动子特性和靶向microRNA，探讨其表达模式和可能的调控机制。结果表明，HMGB 3转录变体1和2（HMGB 3-TV 1和HMGB 3-TV 2）mRNA在乳腺炎感染组织中的表达分别是正常组织的8.46倍和5.31倍（P3中PT SNP影响其碱基启动子活性，不同基因型与牛体细胞数评分相关）。HMGB 3基因的靶基因miR-17- 5 p、miR-20 b和miR-93在乳腺组织中的表达分别是正常乳腺组织的1.56、1.72和2.94倍（P
HMGB3 (high-mobility group box 3) is an X-linked member of a family of sequence-independent chromatin-binding proteins and functions as a universal sentinel for nucleic acidmediated innate immune responses. The splice variant expression, promoter characterization and targeted microRNAs of the bovine HMGB3 gene were investigated to explore its expression pattern and possible regulatory mechanism. The results revealed that the expression of HMGB3 transcript variants 1 and 2 (HMGB3-TV1 and HMGB3-TV2) mRNA in the mastitis-infected mammary gland tissues was up-regulated by 8.46- and 5.31-fold respectively compared with that in healthy tissues (PT SNP in P3 affected its base promoter activity, and different genotypes were associated with the bovine somatic count score. The expression of targets bovine miR-17-5p, miR-20b and miR-93 of the HMGB3 gene was down-regulated 1.56-, 1.72- and 2.94-fold respectively in mammary gland tissues as compared with that in healthy tissues (P