Interaction between the autokinase EpsE and EpsL in the cytoplasmic membrane is required for extracellular secretion in Vibrio cholerae.
Interaction between the autokinase EpsE and EpsL in the cytoplasmic membrane is required for extracellular secretion in Vibrio cholerae.
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细胞质膜中的自激酶 EpsE 和 EpsL 之间的相互作用是霍乱弧菌胞外分泌所必需的。
DOI：
10.1002/j.1460-2075.1995.tb07155.x
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
DiRita,VJ
中科院分区：
文献类型：
--
作者：
Sandkvist,M;Bagdasarian,M;Howard,SP;DiRita,VJ
霍乱弧菌分泌许多对毒力重要的蛋白质，包括霍乱毒素。该过程需要eps基因的产物，其在气单胞菌属、克雷伯菌属和假单胞菌属中具有同源物，并且被认为形成膜相关多蛋白复合物。在这里，我们表明，推定的核苷酸结合蛋白EpsE通过与EpsL相互作用与细胞质膜相关联并由细胞质膜稳定。EpsE和来自嗜水气单胞菌的同源ExeE之间的融合蛋白的分析表明，EpsE的N末端含有EpsL结合结构域，并决定种属特异性。完整的步行者A盒通常存在于ATP结合蛋白中，是体内EpsE活性和体外纯化EpsE自磷酸化所必需的。这些结果表明，激酶活性的EpsE，以及其与推定的细胞质膜蛋白EpsL相互作用的能力所需的毒素易位在霍乱弧菌的外膜。
Vibrio cholerae secretes a number of proteins important for virulence, including cholera toxin. This process requires the products of the eps genes which have homologues in genera such as Aeromonas, Klebsiella and Pseudomonas and are thought to form a membrane‐associated multiprotein complex. Here we show that the putative nucleotide‐binding protein EpsE is associated with and stabilized by the cytoplasmic membrane via interaction with EpsL. Analysis of fusion proteins between EpsE and the homologous ExeE from Aeromonas hydrophila demonstrates that the N‐terminus of EpsE contains the EpsL binding domain and determines species specificity. An intact Walker A box, commonly found in ATP‐binding proteins, is required for activity of EpsE in vivo and for autophosphorylation of purified EpsE in vitro. These results indicate that both the kinase activity of EpsE as well as its ability to interact with the putative cytoplasmic membrane protein EpsL are required for translocation of toxin across the outer membrane in Vibrio cholerae.
“1972政权”日本安保体系下的台湾（中文）
DOI：
--
发表时间：
2022
期刊：
影响因子：
--
作者：
Barrett;B;Ohta;H.;and Trencher;G. "Prospects for Acceleration of Socio-Technical Transitions for Deep Decarbonization," pp. 400-414;青山瑠妙;福田円;舒旻「中国：EUとの脆弱な相互依存」pp. 247-271;福田円;青山瑠妙;青山瑠妙;青山瑠妙;福田円;青山瑠妙;福田円
通讯作者：
福田円
DOI：
--
发表时间：
1994
期刊：
Developments in Plant Pathology
影响因子：
--
作者：
Clarence I. Kado;J. H. Crosa;John F. Fulkerson;Clifford Gabriel;M. Romantschuk;E. Roine;T. Ojanen;Joop van Doorn;J. Louhelainen;Luis Sequeira;K. Klimpel
通讯作者：
K. Klimpel