Effects of telmisartan and linagliptin when used in combination on blood pressure and oxidative stress in rats with 2-kidney-1-clip hypertension
Effects of telmisartan and linagliptin when used in combination on blood pressure and oxidative stress in rats with 2-kidney-1-clip hypertension
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DOI：
10.1097/hjh.0b013e3283649b4d
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发表时间：
2013-11-01
影响因子：
4.9
通讯作者：
Hocher, Berthold
中科院分区：
文献类型：
--
作者：
Chaykovska, Lyubov;Alter, Markus L.;Hocher, Berthold
目的：探讨利格列汀单独及联合血管紧张素II受体阻滞剂（ARB）、替米沙坦对肾血管性高血压大鼠血压、肾功能、心脏形态及氧化应激的影响。将动物随机分为4个给药组（每组n=14-18），接受：替米沙坦（10 mg/kg/天，饮用水）、利格列汀（89 ppm，食物）、复方制剂（利格列汀89 ppm+替米沙坦10 mg/kg/天）或安慰剂。另外一组12只大鼠接受假手术。术后一周测量血压。高血压动物进入16周给药期。在治疗开始后2、4、8、12和16周测量血压。术后6、10、14和18周检测血液和尿液，以评估肾功能和氧化应激。结果：与假手术组相比，肾狭窄导致平均SD收缩压升高（分别为157.7 ± 29.3和106.2 ± 20.5mmHg; P
Objective:To investigate the effects of linagliptin alone and in combination with the angiotensin II receptor blocker (ARB), telmisartan on blood pressure (BP), kidney function, heart morphology and oxidative stress in rats with renovascular hypertension.Methods:Fifty-seven male Wistar rats underwent unilateral surgical stenosis of the renal artery [2-kidney-1-clip (2k1c) method]. Animals were randomly divided into four treatment groups (n=14-18 per group) receiving: telmisartan (10mg/kg per day in drinking water), linagliptin (89ppm in chow), combination (linagliptin 89ppm+telmisartan 10mg/kg per day) or placebo. An additional group of 12 rats underwent sham surgery. BP was measured one week after surgery. Hypertensive animals entered a 16-week dosing period. BP was measured 2, 4, 8, 12 and 16 weeks after the initiation of treatment. Blood and urine were tested for assessment of kidney function and oxidative stress 6, 10, 14 and 18 weeks after surgery. Blood and urine sampling and organ harvesting were finally performed.Results:Renal stenosis caused an increase in meanSD systolic BP as compared with the sham group (157.7 +/- 29.3 vs. 106.2 +/- 20.5mmHg, respectively; P