Serine-arginine protein kinase 1 (SRPK1), a determinant of angiogenesis, is upregulated in prostate cancer and correlates with disease stage and invasion.
Serine-arginine protein kinase 1 (SRPK1), a determinant of angiogenesis, is upregulated in prostate cancer and correlates with disease stage and invasion.
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DOI：
10.1136/jclinpath-2015-203125
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发表时间：
2016-02
影响因子：
3.4
通讯作者：
Oltean S
中科院分区：
文献类型：
--
作者：
Bullock N;Potts J;Simpkin AJ;Koupparis A;Harper SJ;Oxley J;Oltean S
血管内皮生长因子（VEGF）通过选择性剪接产生促血管生成和抗血管生成同种异构体。促血管生成VEGF的优先剪接是由丝氨酸-精氨酸蛋白激酶1 （SRPK1）决定的，SRPK1在许多癌症中上调。在本研究中，我们旨在探讨SRPK1在前列腺癌（PCa）中的表达及其与癌症进展的关系。采用免疫组化方法对110例根治性前列腺切除术患者前列腺癌组织进行SRPK1表达评估。SRPK1在肿瘤组织中的表达明显高于良性组织（p<0.00001），并与pT分期（p=0.004）、囊外延伸（p=0.003）和囊外神经周浸润（p=0.008）相关。有趣的是，表达与Gleason分级无关（p=0.21），这表明SRPK1促进肿瘤微环境的发展，有利于生长和侵袭（可能通过刺激血管生成），而对肿瘤细胞本身的形态或功能几乎没有影响。
Vascular endothelial growth factor (VEGF) undergoes alternative splicing to produce both proangiogenic and antiangiogenic isoforms. Preferential splicing of proangiogenic VEGF is determined by serine-arginine protein kinase 1 (SRPK1), which is upregulated in a number of cancers. In the present study, we aimed to investigate SRPK1 expression in prostate cancer (PCa) and its association with cancer progression. SRPK1 expression was assessed using immunohistochemistry of PCa tissue extracted from radical prostatectomy specimens of 110 patients. SRPK1 expression was significantly higher in tumour compared with benign tissue (p<0.00001) and correlated with higher pT stage (p=0.004), extracapsular extension (p=0.003) and extracapsular perineural invasion (p=0.008). Interestingly, the expression did not correlate with Gleason grade (p=0.21), suggesting that SRPK1 facilitates the development of a tumour microenvironment that favours growth and invasion (possibly through stimulating angiogenesis) while having little bearing on the morphology or function of the tumour cells themselves.