Bound polyphenol extracted from jujube pulp triggers mitochondria-mediated apoptosis and cell cycle arrest of HepG2 cell in vitro and in vivo
Bound polyphenol extracted from jujube pulp triggers mitochondria-mediated apoptosis and cell cycle arrest of HepG2 cell in vitro and in vivo
复制标题
从枣果肉中提取的结合多酚在体内外触发线粒体介导的 HepG2 细胞凋亡和细胞周期停滞
DOI：
10.1016/j.jff.2018.12.017
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发表时间：
2019-02-01
影响因子：
5.6
通讯作者：
Li, Zhuoyu
中科院分区：
文献类型：
--
作者：
Shan, Shuhua;Xie, Yue;Li, Zhuoyu
含有许多养分的中国朱孔有助于健康的饮食。 Jujube纸浆提取物富含具有令人钦佩的药理活性的多酚化合物。目前，从Jujube纸浆中提取的自由和结合的多酚分别命名为JPFP和JPBP。有趣的是，我们发现只有JPBP在HEPG2细胞中表现出显着的抗肿瘤活性，尤其是它在裸小鼠肿瘤模型中也具有相同的作用。此外，该研究表明，p-美乳酸和阿魏酸可能是JPBP中的主要活性成分。从机械上讲，JPBP通过阻断AKT/NF-KAPPA B/BCL-2信号轴来降低线粒体膜电位（MMP）。随后，MMP诱导的线粒体介导的固有凋亡途径和HEPG2细胞中细胞内ROS的产生过多，这导致细胞凋亡。同时，JPBP通过在S期促进HEPG2细胞周期停滞抑制细胞增殖。总的来说，这项研究阐明了JPBP在肝细胞癌的预防和治疗中的潜在应用。
Chinese jujube which contains many nutrients, contributes to a healthy diet. Jujube pulp extracts are rich in polyphenol compounds exhibiting admirable pharmacological activities. In present, free and bound polyphenols extracted from jujube pulp were named JPFP and JPBP, respectively. Interesting, we found only JPBP exhibited prominent anti-tumor activity in HepG2 cells, and especially, it also had the same effect in nude mice tumor model. Further, the study showed that p-Coumaric acid and ferulic acid might be main active components in JPBP. Mechanically, JPBP decreased mitochondrial membrane potential (MMP) via blocking AKT/NF-kappa B/Bcl-2 signal axis. Subsequently, the decrease of MMP induced mitochondria-mediated intrinsic apoptotic pathway and the overproduction of intracellular ROS in HepG2 cells, which contributed to cell apoptosis. Meantime, JPBP suppressed cell proliferation through promoting HepG2 cell cycle arrest in S phase. Collectively, this study sheds light on the potential application of JPBP on the prevention and therapy of hepatocellular carcinoma.