Quantitative and reproducible murine model of excisional wound healing
Quantitative and reproducible murine model of excisional wound healing
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DOI：
10.1111/j.1067-1927.2004.12404.x
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发表时间：
2004-07-01
影响因子：
2.9
通讯作者：
Gurtner, GC
中科院分区：
文献类型：
--
作者：
Galiano, RD;Michaels, J;Gurtner, GC
动物伤口愈合模型的目标是复制人类生理学并预测治疗结果。目前还没有与人类伤口愈合密切平行的啮齿动物伤口愈合模型。啮齿动物是伤口愈合研究的有吸引力的候选者，因为它们的可用性，低成本和易于处理。然而，啮齿类动物模型一直受到批评，因为伤口闭合的主要机制是收缩，而在人类中，上皮再生和肉芽组织形成是涉及的主要机制。本文描述了一种新的模型，在小鼠伤口愈合，利用伤口夹板，是准确的，可重复的，最大限度地减少伤口收缩，并允许伤口愈合发生通过肉芽和上皮再生的过程。我们的研究结果表明，夹板伤口肉芽组织沉积量增加相比，控制，但再上皮化的速度不受影响。因此，该模型消除了伤口收缩，并允许啮齿动物的伤口通过上皮形成和肉芽组织形成而愈合。鉴于这些与人类伤口愈合的类比，我们相信这项技术是研究伤口愈合机制和评估新治疗方式的有用模型。
The goal of animal wound healing models is to replicate human physiology and predict therapeutic outcomes. There is currently no model of wound healing in rodents that closely parallels human wound healing. Rodents are attractive candidates for wound healing studies because of their availability, low cost, and ease of handling. However, rodent models have been criticized because the major mechanism of wound closure is contraction, whereas in humans reepithelialization and granulation tissue formation are the major mechanisms involved. This article describes a novel model of wound healing in mice utilizing wound splinting that is accurate, reproducible, minimizes wound contraction, and allows wound healing to occur through the processes of granulation and reepithelialization. Our results show that splinted wounds have an increased amount of granulation tissue deposition as compared to controls, but the rate of reepithelialization is not affected. Thus, this model eliminates wound contraction and allows rodents' wounds to heal by epithelialization and granulation tissue formation. Given these analogies to human wound healing, we believe that this technique is a useful model for the study of wound healing mechanisms and for the evaluation of new therapeutic modalities.